升清降浊方对慢性肾功能衰竭重要脏器的保护效用及其对炎症因子的影响

PROTECTIVE EFFECT OF ASCENDING QING AND DESCENDING ZHUO PRESCRIPTION ON VITAL ORGANS IN CRF AND ITS INFLUENCE ON INFLAMMATORY FACTORS

目的探讨升清降浊方在延缓慢性肾功能衰竭(CRF)发生发展过程中对重要脏器的保护效用及可能机制。方法180只SPF级SD雄性大鼠被随机分为升清降浊方组、升麻单体治疗组、大黄单体治疗组、基本方治疗组、替米沙坦组和空白组,每组30只。采用大鼠5/6肾切除造模方法制备CRF模型,除空白组不给药物干预外,其余各组连续给药12周。灌药前后采集大鼠血液,检测Scr、BUN、UA等肾功能指标以及C反应蛋白(CRP)、白细胞介素1(IL-1)、白细胞介素6(IL-6)肿瘤坏死因子α(TNF-α)等炎症因子指标的变化;取大鼠肾脏、心脏和肝脏组织观察不同分组各组织的病理改变。结果升清降浊方组、升麻单体治疗组、大黄单体治疗组、基本方治疗组、替米沙坦组大鼠Scr、BUN、UA、CRP、IL-1、IL-6、TNF-α较空白组有明显差异;升清降浊方组、升麻单体治疗组、大黄单体治疗组治疗前后Scr、BUN、UA、CRP、IL-1、IL-6、TNF-α有显著差异;升清降浊方组的Scr、UA、IL-1、IL-6、TNF-α较其它治疗组降低更显著。病理切片结果显示升清降浊方组对大鼠肾脏、心脏及肝脏保护明显优于其他组。结论升清降浊方可能通过调控炎症因子的表达以达到阻断CRF进展,改善重要脏器功能。

Objective:To investigate the protective effects and potential mechanism of ascending qing and descending zhuo prescription in the process of delaying the occurrence and development of CRF.Methods:180 SPF SD male rats were randomly divided into ascending qing and descending zhuo prescription group,rhizoma cimicifugae monotherapy group,rhubarb monotherapy group,basic formula group,telmisartan group and blank group, with 30 rats in each group. 5/6 nephrectomy of rats could be used for establishing CRF model. Exceptfor the blank group, the other groups were given drugs for 12 weeks. Before and after intragastricadministration, blood samples of rats were collected to detect the changes of renal function indicators (SCR,bun and UA) and the inflammatory indexes (CRP, IL-1, IL-6 and TNF-α);the renal, heart and liver tissues ofrats were observed to analyze pathological changes in different groups. Results: There were significantdifferences in Scr, BUN, UA, CRP, IL-1, IL-6 and TNF-α of the rats from ascending qing and descending zhuoprescription group, rhizoma cimicifugae monotherapy group, rhubarb monotherapy group, basic formula groupand telmisartan group, compared with that of blank group;Scr, BUN, UA, CRP, IL-1, IL-6 and TNF-α ofascending qing and descending zhuo prescription group, rhizoma cimicifugae monotherapy group and rhubarbmonotherapy group were notably different before and after treatment;SCR, UA, IL-1, IL-6 and TNF–α inascending qing and descending zhuo prescription group were significantly lower than those in other groups. Thepathological section showed that the protection of kidney, heart and liver in ascending qing and descendingzhuo prescription group was better than that in other groups. Conclusion: Ascending qing and descending zhuoprescription could regulate the expression of inflammatory factors to block the progress of CRF and improvevital organ functions.