牡蛎原肌球蛋白的提取工艺优化

Optimization of extraction process of oyster promyosin

目的探究牡蛎原肌球蛋白提取的最优工艺。方法以养殖牡蛎为原料,探究料液比、提取次数、提取温度、pH等单因素对牡蛎原肌球蛋白提取率的影响,并通过正交实验对其进行优化。结果在pH5.5的基础上得出牡蛎原肌球蛋白提取的最优工艺参数是:料液比为1:2、提取次数为6次、提取温度为100℃,此时原肌球蛋白的得率为6.53%。结论本研究优化的方法大大提高了牡蛎原肌球蛋白的提取率。

Objective To explore the optimal technology for extraction of oyster tropomyosin.Methods Taking cultured oyster as raw material,the influences of single factors such as solid-liquid ratio,extraction times,extraction temperature and pH on the extraction rate of tropomyosin from oyster were investigated,and optimized by orthogonal experiment.Results On the basis of pH 5.5,the optimal technological parameters for the extraction of oyster tropomyosin were as follows:The ratio of solid to liquid was 1:2,the extraction times were 6,and the extraction temperature was 100℃.At this time,the yield of tropomyosin was 6.53%.Conclusion The optimized method in this study has greatly improved the extraction rate of tropomyosin from oyster.