两种不同光热转化剂体外治疗口腔鳞状细胞癌的疗效差异对比研究

Comparative study on the therapeutic effect of two photothermal conversion agents on oral squamous cell carcinoma in vitro

目的探究两种不同新型光热转化剂的光热性能及其光热体外治疗口腔鳞状细胞癌的疗效差异,为口腔鳞癌光热治疗提供材料基础。方法制备亲水性的光热转化剂超小型硫化铜纳米颗粒(ultra⁃small Cu2-x S nanoparticles,Cu2-xS NPs)和四氧化三铁纳米颗粒(Fe3O4 nanoparticles,Fe3O4 NPs),记录Cu2-xS NPs和Fe3O4 NPs溶液升温和升温-降温循环温度变化,酶标仪记录光热治疗后口腔鳞癌OSC⁃19细胞活性。结果成功合成Cu2-xS NPs和Fe3O4 NPs;近红外(near⁃infrared,NIR)激光照射下,二者溶液温度升高,Cu2-xS NPs溶液温度升高更明显,二者升温-降温循环稳定;Cu2-xS NPs和Fe3O4NPs没有明显的细胞毒性,介导的光热治疗可以降低OSC⁃19细胞的活力至35.35%(Cu2-xS NPs)和50.76%(Fe3O4NPs)。结论Cu2-xS NPs和Fe3O4 NPs具有良好的光热性能和生物相容性,可以对口腔鳞癌进行体外光热治疗,Cu2-xSNPs对口腔鳞癌的体外光热治疗效果强于Fe3O4NPs,最终为口腔鳞癌的光热治疗提供材料依据。

Objective To investigate the photothermal properties of two different new types of photothermal conversion agents and the difference in their effects on oral squamous cell carcinoma in vitro treatment,and provide a material basis for photothermal therapy of o⁃ral squamous cell carcinoma.Methods Hydrophilic ultra⁃small Cu2-xS nanoparticles(Cu2-xS NPs)and Fe3O4 nanoparticles(Fe3 O4 NPs)were synthesized.The temperature changes of Cu2-xS NPs and Fe3O4 NPs were monitored throughout heating and heating⁃cooling process.The viability of OSC⁃19 cells was recorded by microplate reader.Results Cu2-xS NPs and Fe3O4 NPs were successfully syn⁃thesized;under NIR laser,the temperature of both agents’solution increased,while the temperature of Cu2-xS NPs solution increased more obviously,and the heating⁃cooling cycles of them were stable.Cu2-xS NPs and Fe3O4 NPs had no obvious cytotoxicity,and could reduce the viability of OSC⁃19 cells to 35.35%(Cu2-xS NPs)and 50.76%(Fe3 O4 NPs).Conclusion Cu2-x S NPs and Fe3 O4 NPs have excellent photothermal effects and biocompatibility.They can be used for in vitro photothermal therapy of oral squamous cell carci⁃noma.The effect of Cu2-xS NPs on the in vitro photothermal therapy of oral squamous cell carcinoma is stronger than that of Fe3O4 NPs,which may provide a material foundation for photothermal therapy.