超声-微波协同辅助提取桑葚花色苷及抗氧化性研究

Optimization of Ultrasonic-microwave Assisted Extraction of Anthocyanins from Mulberry and Their Antioxidant Activities

采用超声-微波协同技术提取沧州地区桑葚中的花色苷,对其抗氧化性进行分析。以花色苷提取率为优化指标,通过单因素试验和响应面法优化得到超声-微波协同技术提取花色苷的最佳工艺为:提取剂浓度70%、料液比3∶90(g/mL)、微波功率150 W、超声波功率360 W,在此条件下,花色苷的提取率最高,为(13.57±1.3)mg/g。抗氧化性研究结果表明:花色苷提取液清除羟自由基和DPPH自由基的能力远高于相同浓度的Vc,具有较好的抗氧化活性。

The anthocyanins are extracted from mulberries in Cangzhou area by ultrasonic-microwave collaborative technology and their antioxidant activities are analyzed.With the extraction rate of anthocyanins as the optimal index,the optimal process for the extraction of anthocyanins by ultrasonic-microwave collaborative technology is obtained through single factor test and response surface method optimization:the extractant concentration is 70%,the solid-liquid ratio is 3∶90(g/mL),the microwave power is 150 W and the ultrasonic power is 360 W.Under the optimum conditions,the extraction rate of anthocyanins is(13.57±1.3)mg/g.The results of antioxidant activity show that the scavenging activity of anthocyanin extract to hydroxyl radical and DPPH radical is much higher than that of Vc with the same concentration,and has better antioxidant activity.