不同外源酶组合对棕榈粕原料体外养分消化率和酶水解物能值的影响

Effects of Different Combinations of Exogenous Enzymes on in Vitro Nutrient Digestibility and Enzymatic Hydrolyzate Gross Energy of Palm Kernel Meal Ingredients

本试验旨在利用单胃动物仿生消化系统(SDS-Ⅱ)探究不同外源酶组合对棕榈粕原料体外干物质消化率(IVDMD)、体外粗蛋白质消化率(IVCPD)、体外能量消化率(IVGED)和酶水解物能值(EHGE)的影响,以评价不同来源棕榈粕[马来西亚棕榈粕(MPK)和菲律宾棕榈粕(PPK)]的营养价值并筛选对应的最优外源酶组合。试验1采用2×3因子设计,设6个组,即分别以不添加外源酶的PPK和MPK原料作为对照组,以及分别向PPK原料中添加酶包-A(EP-A)、酶包-B(EP-B)和分别向MPK原料中添加EP-A、EP-B作为试验组。利用SDS-Ⅱ模拟猪全消化道消化过程,测定各组的IVDMD、IVCPD、IVGED和EHGE。试验2采用单因素完全随机设计,设5个组(EP0、EP1、EP2、EP3和EP4组),以不添加外源酶的EP0组为对照组,试验组分别在MPK原料中添加不同的外源酶组合,分别测定各组的IVDMD、IVCPD、IVGED和EHGE,以筛选最优外源酶组合。2个试验中每个样品均设5个重复,每个重复设1根消化管。结果显示:1)PPK的IVDMD、IVCPD、IVGED均显著高于MPK(P<0.05),PPK的EHGE与MPK相比无显著差异(P>0.05)。2)添加外源酶组合EP-A和EP-B均能显著提高MPK和PPK的IVGED和EHGE(P<0.05),添加外源酶组合EP-A和EP-B均能显著提高MPK的IVCPD(P<0.05),添加外源酶组合EP-B能显著提高MPK和PPK的IVDMD(P<0.05)。3)添加不同外源酶组合均能显著提高MPK的IVDMD、IVCPD、IVGED和EHGE(P<0.05),且EP4组的IVDMD、IVGED和EHGE显著高于EP1、EP2和EP3组(P<0.05)。由此可见,PPK的IVDMD、IVCPD、IVGED均高于MPK,为更加优质的棕榈粕来源;添加外源酶组合EP-A和EP-B均可提高2种来源棕榈粕的IVDMD、IVCPD、IVGED和EHGE,且EP-B效果更佳;EP4组的外源酶组合为酶解MPK的最优组合。

This experiment was conducted to investigate the effects of different combinations of exogenous enzymes on in vitro dry matter digestibility(IVDMD),in vitro crude protein digestibility(IVCPD),in vitro gross energy digestibility(IVGED)and enzymatic hydrolyzate gross energy(EHGE)of palm kernel meal ingredients using a computer-controlled simulated digestion system(SDS-Ⅱ),and to evaluate the nutritional value of palm kernel meal from two sources[Malaysia palm kernel cake(MPK)and Philippines palm kernel cake(PPK)]and screen the corresponding optimal exogenous enzyme combination.In experiment 1,a 2×3 factor design was adopted,consisted of 6 groups,in which the control groups were MPK and PPK ingredients without exogenous enzymes addition,and the experimental groups were supplemented exogenous enzyme package-A(EP-A),exogenous enzyme package-B(EP-B)in PPK ingredient and supplemented EP-A,EP-B in MPK ingredient,respectively.Using SDS-Ⅱto simulate the total tract digestive process of pigs,the IVDMD,IVCPD,IVGED and EHGE of each groups were determined.In experiment 2,a single factor completely random design adopted,consisted of 5 groups(EP0,EP1,EP2,EP3 and EP4 groups),in which the control group(EP0 group)were without exogenous enzymes addition,and the experimental groups were supplemented different combinations of exogenous enzymes in MPK ingredient.The IVDMD,IVCPD,IVGED and EHGE of each groups were determined.Five replicates per sample and one digestive tract for each replicate were set in both experiments.The results showed as follows:1)the IVDMD,IVCPD and IVGED of PPK were significantly higher than those of MPK(P<0.05),but the EHGE of PPK had no significant difference with that of MPK(P>0.05).2)The supplementation of exogenous enzyme combinations EP-A and EP-B significanltly increased the IVGED and EHGE of MPK and PPK(P<0.05),the supplementation of exogenous enzyme combinations EP-A and EP-B significanltly increased the IVCPD of MPK(P<0.05),and the supplementation of exogenous enzyme combination EP-B significanltly increased the IVDMD of MPK and PPK(P<0.05).3)The supplementation of different combinations of exogenous enzymes significanltly increased the IVDMD,IVCPD,IVGED and EHGE of MPK(P<0.05),and the IVDMD,IVGED and EHGE of EP4 group were significantly higher than those of EP1,EP2 and EP3 groups(P<0.05).In conclusion,the IVDMD,IVCPD and IVGED of PPK are higher than those of MPK,which is a better source of palm kernel meal;the supplementation of exogenous enzyme combinations EP-A and EP-B can increase the IVDMD,IVCPD,IVGED and EHGE of palm kernel meal from two sources of which EP-B has better effects;the exogenous enzyme combination of EP4 group is the optimal combination for MPK enzymolysis.