加味生脉散对飞碟运动员高温训练前后外周血中血液生化及HSP72表达的影响

Effect of Shengmaisan on the Expression of Blood and Blood biochemical Index and Heat Shock Protein 72 in Flying-Saucer-Shooter Trained under High Temperature

目的:研究加味生脉散对飞碟运动员高温训练前后外周血中热休克蛋白72(HSP72)的表达和血液生化的影响,为防热疾病药物增强飞碟运动员热耐力、预防热疾病提供实验依据。方法:选取浙江省飞碟运动员24人,采用随机数字表方法分为对照组、中药组两组,每组12人。其中,未饮用加味生脉散者为对照组,饮用加味生脉散者为中药组。在夏训期间,进行高温训练任务2周,中药组飞碟运动员每日上、下午训练前饮用加味生脉散,实验前及2周后分别抽取每组外周静脉血,分别进行淋巴细胞HSP72含量、血液常规及生化指标测定。结果:实验前,经测定,对照组、中药组的血液常规、生化检测值、HSP72表达均无异常,经比较,无显著差异(P>0.05)。高温训练结束后,中药组的HSP72表达显著高于训练前及对照组(P<0.05),白细胞计数实验前后存在显著差异(P<0.05)。结论:服用加味生脉散能显著提高飞碟运动员受热刺激后HSP72表达的上调,达到增强热耐受性、预防热疾病的作用。

Objective To study the effects of Jiawei Shengmaisan on the expression of heat shock protein 72(HSP72)in peripheral blood lymphocyte and blood biochemistry of flying saucer athletes before and after high temperature training,so as to provide experimental basis for anti-heat drugs to enhance the thermal endurance of flying saucer athletes and prevent heat diseases.Methods Twenty-four flying saucer athletes in Zhejiang Province were selected and randomly divided into two groups,12 in each group.Those who did not drink the Jiawei Shengmaisan were the control group,while those who drank the Jiawei Shengmaisan were the experimental group.During the 2 weeks of high temperature training task,the experimental group drank Jiawei Shengmaisan daily.Peripheral venous blood of each group was taken before and after the experiment.The HSP72 content of lymphocyte,blood routine and biochemical indexes were measured respectively.Results Before the experiment,there was no significant difference in the blood routine biochemical test values of the two groups and the expression of HSP72 between the experimental group and the control group(P>0.05).After the experiment,the expression of HSP72 in the experimental group was significantly higher than that before the training and the control group(P<0.05);there was significant difference in white blood cell count before and after the experiment.Conclusion Jiawei Shengmaisan can improve the ability of flying saucer athletes to express HSP72 after being stimulated by heat,thus enhancing the thermal endurance and preventing heat diseases.