UPLC-MS/MS法同时测定黄芪-甘草药对8个有效成分

Simultaneous determination of eight active components in Astragali Radix and Glycyrrhizae Radix by UPLC-MS/MS

建立UPLC-MS/MS方法同时测定黄芪-甘草药对中毛蕊异黄酮葡萄糖苷、甘草苷、芒柄花苷、甘草素、毛蕊异黄酮、黄芪甲苷、芒柄花素、甘草酸8种主要成分.采用色谱柱为Acquity BEH C 18(50 mm×2.1 mm,1.7μm)柱,流动相为乙腈-体积分数0.1%乙酸水溶液洗脱系统,流速为0.3 mL·min^-1,采用ESI离子源,MRM模式检测.结果显示,所测8种主要成分在测定范围内线性关系良好,相关系数均大于0.9991;精密度、重复性和稳定性良好;平均加样回收率为96.63%~99.95%,RSD≤3.48%.首次建立可同时测定黄芪-甘草药对中8种主要成分的UPLC-MS/MS方法,该法操作简便,灵敏度高,专属性好,分析速度快,为解析黄芪-甘草药对的临床应用提供科学依据.

To establish a method for the simultaneous determination of eight active component(calycosin-7-glucoside, liquiritin, ononin, liquiritigenin, calycosin, astragaloside Ⅳ,formononetin,and glycyrrhizinic acid) in the Astragali Radix-Glycyrrhizae Radix herb pair.The determination was performed on an Acquity BEH C18(50 mm × 2.1 mm,1.7 μm),and the mobile phase was composed of 0.1% acetic acid in water and acetonitrile in gradient elution and at 0.3 mL·min-1 flow rate.Mass spectrometer with electro spray ionization(ESI) source was operated in MRM mode.All of the analytes showed good linearity(r≥0.9991) in the tested ranges.The precision,repeatability and stability of the method were good for the eight components.The average recoveries were in the range of 96.63%-99.95% with relative standard deviations(RSD) ≤3.48%.A simple,accurate,and sensitive method was established for quantitative analysis of 8 active components in the Astragali Radix-Glycyrrhizae Radix herb pair.These findings will provide helpful information for the clinical administration of the Astragali Radix-Glycyrrhizae Radix herb pair.