f101基因3’端非翻译区微小RNA结合位点单核苷酸多态性与非霍奇金淋巴瘤患者预后的关系

Single nucleotide polymorphism at the microRNA binding site in the 3’-untranslated region of the f101 gene and prognosis of non-Hodgkin lymphoma

目的探讨微小(miRNA)结合位点f101基因的3’端非翻译区的单核苷酸多态性(SNP)对非霍奇金淋巴瘤(NHL)患者癌症风险和临床预后的影响。方法miRNA结合位点f101基因的3’端非翻译区rs4901706 SNP基因分型采用连接酶检测反应。rs4901706与NHL生存相关性采用log-rank检验及Kaplan-Meier方法。多因素生存分析采用Cox比例风险模型。结果携带rs4901706 AA基因型患者比携带AG/GG基因型患者生存时间长(P=0.017)。多因素分析结果表明,rs4901706 SNP与NHL患者总生存率独立相关(相对风险:1.768,95%CI:1.045~2.997,P=0.035)。rs4901706 SNP与NHL生存相关性在T细胞淋巴瘤亚型也得到验证(P=0.025)。结论f101基因3’端非翻译区miRNA结合位点SNP可能影响NHL患者的生存,对临床预后有预测意义。

Objective The microRNA(miRNA)binding-site single nucleotide polymorphism(SNP)rs4901706 located in the 3’-untranslated region(UTR)of f101 was genotyped in non-Hodgkin lymphoma(NHL)patients to assess their relationship with cancer risk and overall survival.Methods The miRNA binding site SNP of rs4901706 in the 3’-UTR of f101 was genotyped using the ligation detection reaction method.The association of rs4901706 in f101 with NHL survival was calculated with the log-rank test using the Kaplan-Meier method.Multivariate survival analysis was performed using a Cox proportional hazards model.Results Patients with the rs4901706 AA genotype presented a significantly longer survival time than patients with the AG/GG genotype(P=0.017).In addition,the results of the multivariate analysis revealed that the rs4901706 SNP was independently associated with the survival of NHL patients(RR:1.768,95%CI:1.045-2.997,P=0.035).Furthermore,the statistically significant association between the rs4901706 SNP and NHL survival was corroborated in the T-cell lymphoma(TCL)subtype of NHL(P=0.025).Conclusion Our study proved that the SNP at the miRNA binding site in the f1013’-UTR is influential in determining the survival rate of patients with NHL.Thus,it may have a promising clinical prognostic and survival significance.