三氟乙酸增强的解吸电喷雾电离质谱成像用于脂质空间分布研究

Investigation of Lipid Distributions by Trifluoroacetic Acid-enhanced Desorption Electrospray Ionization Mass Spectrometry Imaging

将三氟乙酸添加到解吸电喷雾电离质谱的喷雾溶剂中,提高了对脂质的成像检测能力,使小鼠脑组织切片中能检测到的脂质种类从59种提高到71种。此外,该方法还可以促进脂质的质子化峰的形成并抑制钠和钾加合物的形成,有利于简化谱图解析过程并实现更好的定量分析,利用该方法实现了小鼠脑组织切片上多种脂质的空间相关性研究,为探索脂质间潜在的代谢途径提供了新手段,在神经学、临床等领域具有巨大的应用前景。

A large number of lipids play an important role in the process of life,so it is important to simultaneously visualize multiple lipids by mass spectrometry imaging.By adding trifluoroacetic acid into the spray solvent of desorption electrospray ionization(DESI),the number of detectable lipids was increased from 59 to 71 in the mouse brain tissue sagittal section.This method could not only improve the detection sensitivity,but also suppress the formation of sodium and potassium adducts of lipids,to simplify spectrum interpretation and facilitate quantitative analysis.Furthermore,the result also revealed the spatial correlation among the lipids in the whole mouse brain tissue section as well as specific brain regions.The present protocol provides a new research method for exploring potential metabolic pathways between lipids,suggesting the potential application in neuroscience,clinical,and other fields.