摘要
2018年11月—2019年3月,在海南省三亚、乐东、东方、儋州、澄迈、海口、万宁和五指山共8个市县,采集疑似病毒病的叶片样本302份,采用小RNA深度测序和RT-PCR检测发现,黄瓜样品中存在黄瓜花叶病毒(Cucumber mosaic virus,CMV)、甜瓜黄斑病毒(Melon yellow spot virus,MYSV)、黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)、烟草花叶病毒(Tobacco mosaic virus,TMV)和瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus,CCYV)共5种病毒,检出率分别为32.79%、31.46%、23.18%、9.61%和2.65%。病毒的复合侵染率为11.28%,有8种复合侵染类型,以其中2种病毒复合侵染为主。明确了海南岛黄瓜病毒病主要种类及发生分布情况。
From November 2018 to March 2019,a total of 302 suspected virus-infected cucumber leaf samples were collected from 8 regions(Sanya,Ledong,Dongfang,Danzhou,Chengmai,Haikou,Wanning and Wuzhishan)in Hainan Island.Five viruses were detected including Cucumber mosaic virus(CMV),Melon yellow spot virus(MYSV),Cucumber green mottle mosaic virus(CGMMV),Tobacco mosaic virus(TMV),and Cucurbit chlorotic yellows virus(CCYV)by small RNA deep sequencing and RT-PCR.The detection rate was 32.79%,31.46%,23.18%,9.61%and 2.65%,respectively.Detection rate of co-infection was 11.28%.There were 8 kinds of co-infection types,and the co-infection of two viruses occurred most often.The distribution and species of viruses from cucumber in Hainan were clarified.
作者
车海彦
曹学仁
贺延恒
罗大全
CHE Haiyan;CAO Xueren;HE Yanheng;LUO Daquan(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture and Rural Affairs/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第11期2280-2284,共5页
Chinese Journal of Tropical Crops
基金
海南省重大科技计划项目(No.zdkj201813)
中国热带农业科学院基本科研业务费专项(No.19CXTD-33)。
关键词
海南岛
黄瓜病毒病
小RNA深度测序
病原鉴定
分布
Hainan Island
cucumber virus disease
small RNA deep sequencing
pathogen identification
distribution
