髓源性生长因子对糖尿病小鼠BMSCs骨向分化的影响及机制研究

The effects of myeloid-derived growth factor on osteoblast differentiation of BMSCs from diabetic mice and the related mechanisms

目的:探讨髓源性生长因子(MYDGF)对糖尿病小鼠骨髓间充质干细胞(BMSCs)成骨分化的影响及其信号机制。方法:分离、培养糖尿病小鼠BMSCs,碱性磷酸酶(ALP)活性检测BMSCs的成骨活性,茜素红染色观察钙结节,实时荧光定量PCR(qPCR)检测成骨相关基因Runx2、Osx、ALP和OCN的表达,Western blot检测PI3K、Akt的磷酸化水平。结果:MYDGF呈浓度依赖性地增强ALP活性,促进钙结节形成,显著上调Runx2、Osx和ALP的表达(P<0.05),显著提高PI3K和Akt的磷酸化水平(P<0.05)。结论:MYDGF可通过激活PI3K/Akt信号通路促进糖尿病小鼠BMSCs骨向分化。

Objective:To investigate the effects of myeloid-derived growth factor(MYDGF)on osteoblast differentiation of bone marrow mesenchymal stem cells(BMSCs)from diabetic mice and the related signaling mechanisms.Methods:BMSCs from diabetic mice were in vitro cultured in osteogenic differentiation medium,ALP activity was tested,qRT-PCR was used to evaluate the expression levels of Runx2,Osx,ALP and OCN,phosphorylation level of PI3K and Akt was detected by Western blot.Results:MYDGF promoted ALP activity in a concentration-dependent manner,increased mineralized nodules,significantly upregulated mRNA expression of Runx2,Osx and ALP(P<0.05);significantly increased phosphorylation level of PI3K and Akt(P<0.05).Conclusion:MYDGF promotes osteoblast differentiation of BMSCs from diabetic mice by activating PI3K/Akt signaling pathway.