叶酸水分检测的研究

Study on the Determination of Folic Acid Moisture

通过卡尔费休法分萃取时间与其对叶酸的水分检测结果的影响关系进行研究,优化卡尔费休法测定水分的方法。方法:万通915卡氏水分仪“停止判据”设置为“漂移”,“停止漂移”为20μL/min,“萃取时间”设置为0~400s;“漂移校正”设置为自动。萃取时间在50~200s内,随着萃取时间的延长,检测出的水分含量提高,检测的准确性增加;萃取时间在200~350s内,随着萃取时间的延长,检测出的水分含量随着时间增加以恒定的速率不显著的提高,检测的准确性有下降趋势。经验证文章建立的卡尔费休法方法测定水分的最佳萃取时间为200s,为叶酸对照品的标定及其原料的质量控制提供了依据。

To study the relationship between the extraction time of Karl Fischer method and its influence on the results of folic acid moisture determination,and optimize the method of Karl Fischer method to determine moisture.The“Stop Criterion”of Metrohm 915 Karfelter Moisture Analyzer is set to“Drift”,“Stop Drift”is set to 20μL/min,“Extraction Time”is set to 0~400 s;“Drift Correction”is set to automatic.The extraction time is within 50~200s.With the extension of the extraction time,the detected moisture content increases and the accuracy of detection increases;The extraction time is within 200~350s,with the extension of the extraction time,the detected moisture content increases by not significantly growth rate,and the accuracy of detection has a downward trend.The best extraction time of Karl Fischer method is 200s for water determination in the research,which provides a basis for the calibration of folic acid reference substance and the quality control of raw materials.