河北地区丙肝感染患者丙型肝炎病毒基因型分布特征

HCV Genotype and Subtype Distribution of Patients with Chronic Hepatitis C in Hebei Area

目的了解河北地区丙肝感染患者丙型肝炎病毒(HCV)基因型分布情况、感染途径、基因亚型与性别、年龄的关系,并与其他省份的HCV基因型分布模式进行比较。方法收集284例HCV感染患者血清样本,对其进行HCV-RNA定量检测和HCV基因分型检测。结果在284例临床样本中共检测出4种基因型(1~3和6)和5种亚型,分别是1b型(n=191,67.25%)、2a型(n=90,31.69%)、3a型(n=1,0.35%)、3b型(n=1,0.35%)和6a型(n=1,0.35%),未发现亚型混合感染。结论河北地区HCV感染基因型以1b型为主,其次为2a型。HCV感染率随年龄的变化呈现不同的变化,50~59岁年龄组的感染者人数最多,且女性感染者的数量显著高于男性(P<0.05)。经文献比较发现,河北省虽然地处华北地区,但其HCV基因型分布模式与中国东北地区相似。

Objective To investigate the distribution of HCV prevalence and genotypes among different gender and age in Hebei area,and analyze the transmission routes of HCV.The genotype distribution pattern was compared with those of neighboring regions in China.Methods A total of 284 patients were analyzed by RT-PCR and DNA sequencing.Results In total,four HCV genotypes and five subtypes were identified among 284 patients:1b(n=191,67.25%),2a(n=90,31.69%),3a(n=1,0.35%),3b(n=1,0.35%)and 6a(n=1,0.35%)respectively.Mixed infections of the dominant subtypes were not found.Conclusion Hepatitis C virus infection in Hebei Area is dominated by type 1b,followed by type 2a.This article revealed the comprehensive distribution patterns of HCV genotypes in Hebei Area.The prevalence of HCV infection showed different variations with age,as the highest incidence was shown for the age group 50~59.Though Hebei Province was located in north China,its HCV distribution pattern was more similar to those of the regions in northeast China.