河豚鱼皮胶原肽螯合锌的组成与急性毒性研究

Study on the composition and acute toxicity of collagen peptide chelated zinc from puffer skin

对河豚鱼皮胶原肽螯合锌的主要成分、分子质量分布及氨基酸组成作了分析和比较,并通过急性毒性试验,初步评价其毒理学安全性。实验结果表明,河豚鱼皮胶原肽螯合锌的蛋白含量为(82.04±0.68)%,锌含量为(12.86±1.24)%,胶原肽的分子质量主要集中在600 Da附近。氨基酸分析显示,河豚鱼皮胶原肽螯合锌含有丰富的甘氨酸、羟脯氨酸以及大量的天冬氨酸和谷氨酸,基质辅助激光解吸电离飞行时间质谱图显示了肽-锌螯合物的生成。通过急性毒性试验,得出河豚鱼皮胶原肽螯合锌的半数致死量为6.8479 g/kg,95%的可信限为6.1224~7.6765 g/kg,根据急性毒性剂量分级标准,属于实际无毒级。

In this paper,the main components,molecular weight distribution and amino acid composition of collagen peptide chelated zinc from puffer skin were analyzed and compared.Its toxicological safety was preliminarily evaluated by acute toxicity test.The results showed that the protein content of collagen peptide chelated zinc from pufferfish skin was(82.04±0.68)%,and the zinc content was(12.86±1.24)%.The molecular weight of collagen peptides from puffer skin was mainly concentrated around 600 Da.Amino acid analysis indicated collagen peptide chelated zinc from puffer skin contained rich glycine,hydroxyproline,aspartic acid and glutamate.The MALDI-TOF mass spectrometry showed the generation of the peptide-zinc chelate.Through the acute toxicity test,the LD 50 of collagen peptide chelated zinc from puffer skin was 6.8479 g/kg,and the 95%confidence limit was 6.1224~7.6765 g/kg.According to the acute toxicity dose grading standard,the collagen peptide chelated zinc from puffer skin was actually non-toxic.