摘要
纤维二糖差向异构酶(cellobiose 2-epimerase)是近年来颇受关注的一种工业酶,主要应用于乳果糖(lactulose)的生物法制备,也适用于D-甘露糖的生产。全球年需求量近10万t,具有较高的商业价值,其中乳果糖可用于治疗便秘和肝性脑病,是一种常见的非处方药,效果显著。该文针对纤维二糖差向异构酶制备乳果糖工艺的酶源挖掘、蛋白质工程改造、固定化酶研究等方面进行了综述,为制备乳果糖的理论和应用研究提供参考。
Cellobiose 2-epimerase has attracted growing attentions as an industrial enzyme.It was applied to lactulose bio-production,as well as D-mannose preparation.Lactulose was used for treating constipation and hepatic encephalopathy as a common over-the-counter drug,and showing a high efficiency.The annual global demand for lactulose was nearly 100000 tons,showing great commercial value.In this review,recent advances in new enzyme characterization,protein engineering and enzyme immobilization for cellobiose 2-epimerase are summarized,which would shed light on researches in this field.
作者
徐铮
诸亚锋
XU Zheng;ZHU Yafeng(College of Food Science and Light Industry,Nanjing Technology University,Nanjing 211816,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第23期248-254,共7页
Food and Fermentation Industries
基金
江苏省六大人才高峰项目(NY-056)。
