肝部肿瘤微波消融对患者外周血T细胞亚群的影响

Effect on T Cells Subsets in Peripheral Blood Caused by Microwave Ablation Against Hepatic Malignance

目的探讨肝部肿瘤微波消融(MWA)对患者外周血T细胞亚群的影响。方法于消融前、消融后24 h、消融后15 d、消融后30 d采集患者外周血。应用流式细胞术分析外周血CD3^+,CD3^+/CD4^+,CD3^+/CD8^+细胞比例并计算对比CD4^+/CD8^+比值。采用配对样本t检验对数据进行分析。结果共纳入肝恶性肿瘤行MWA治疗患者43例,在微波消融后15 d,CD3^+,CD3^+/CD4^+和CD3^+/CD8^+细胞比率显著增高。在微波消融后30 d,仅CD3^+细胞显著增加,其在消融后24 h未发现显著差异。结论肝部肿瘤MWA可能在短期内影响患者的免疫功能状态。

Objective To investigate the effects of microwave ablation on T-lymphocyte subsets in patients underwent microwave ablation against hepatic malignance.Methods Peripheral blood was collected from patients with hepatic malignance treated by microwave ablation.T cell subsets(CD3^+,CD3^+/CD4^+and CD3^+/CD8^+cells),were analyzed by flow cytometry.The comparison timings were:before ablation,24 hours after ablation,15 days after ablation,and 30 days after ablation.Data was analyzed using paired sample t-test.Results A total of 43 patients with hepatic malignancies were recorded.For the T cell subsets,significant increases were found on CD3^+,CD3^+CD4^+,and CD4^+/CD8^+ratio 15 days post-microwave ablation.Only CD3^+cell show significant increase 30 days post-microwave ablation,but no significant difference was found 24 hours after the ablation.Conclusion MWA can improve the immune function status of patients with hepatic malignancies.