HPV E6/E7检测在宫颈病变筛查中的应用价值

Application Values of HPV E6/E7 Detection in Cervical Lesion Screening

目的探讨人乳头状瘤病毒(human papillomavirus,HPV)E6/E7检测在宫颈病变筛查中的应用价值。方法选择进行宫颈鳞状上皮内病变(cervical intraepithelial neoplasia,CIN)筛查的患者62例,所有患者都给予HPV DNA、E6/E7 mRNA检测,采用免疫组化方法检测p53与ki-67表达情况。结果CINⅠ级、Ⅱ级、Ⅲ级、宫颈癌的HPV DNA阳性率分别为33.3%、77.8%、88.9%和100.0%;HPV E6/E7 mRNA阳性率分别为33.3%、83.3%、88.9%和100.0%,P53、Ki67阳性率分别为30.0%、66.7%、88.9%、100.0%和33.3%、72.2%、77.8%和100.0%,对比差异都有统计学意义(P<0.05)。在62例患者中,Pearson相关分析显示HPV E6/E7 mRNA阳性率都与HPV DNA、P53、Ki67阳性率呈现显著正相关性(P<0.05)。结论随着宫颈鳞状上皮内病变级别的增加,HPV E6/E7 mRNA阳性率增加,也伴随有HPV DNA、P53、Ki67阳性率增加,两者具有相关性。

Objective To explore the application values of human papillomavirus(HPV)E6/E7 detection in cervical lesion screening.Methods 62 cases of patients with cervical squamous intraepithelial neoplasia(CIN)were selected for screening.All patients were tested for HPV DNA and E6/E7 mRNA,and used the immunized histochemical methods were used to detect the expression of p53 and ki-67.Results The positive rates of HPV DNA in CIN gradesⅠ,Ⅱ,Ⅲ,and cervical cancer were 31.9%,75.6%,95.0%and 100.0%;HPV E6/E7 mRNA positive rates were 33.3%,80.5%,90.0%and 100.0%,the positive rates of P53 and Ki67 were 31.9%,68.3%,85.0%,100.0%and 33.3%,70.0%,80.8%and 100.0%,respectively,and compared the differences were statistically significant(P<0.05).In the 62 patients,Pearson correlation analysis showed that HPV E6/E7 mRNA positive rates were significantly positively correlated with HPV DNA,P53,and Ki67 positive rates(P<0.05).Conclusion As the level of cervical squamous intraepithelial lesions increases,the HPV E6/E7 mRNA positive rate increases,which are also accompanied by the increase of HPV DNA,P53,and Ki67 positive rates.