期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

基于多肽标记物分析的肉制品掺伪定量检测技术 被引量：1

Quantitative Method for Adulterated Meat Products Based on Peptide Marker Analysis
下载PDF
导出
摘要 该文开发了一种基于多肽标记物分析的肉制品掺伪检测技术,可同时进行定性和定量分析。样品经蛋白质提取和胰蛋白酶消化后,采用高分辨质谱(HRMS)和生物信息学工具,寻找牛、鸡、鸭、猪和羊5种肉类的特异性热稳定多肽标记物。在多反应监测(MRM)模式下,通过高效液相色谱-串联四极杆质谱(HPLC-QQQ-MS)对这些标记物进一步验证。使用由两种不同质量分数的肉类混合物(0%~100%)建立定量校准曲线,测定低限可达1%。采用该方法对市售的实际样品进行测试,验证结果表明该方法是一种可靠、有效的肉制品鉴定手段,可同时用于生肉和熟肉掺伪的定量检测。 A detecting method for adulteration in processed meat products based on peptide markers analysis was developed in this paper,which could be performed for both qualitative and quantitative analysis.After protein extraction and trypsin digestion,the specific heat-stable peptide markers were searched in five types of meat including cattle,chicken,duck,pig and sheep,with high-resolution mass spectrometry(HRMS)and bioinformatics tools.In multiple reaction monitoring(MRM)mode,these markers were further verified by high performance liquid chromatography triple quadrupole mass spectrometry(HPLC-QQQ-MS).A quantitative calibration curve was established using a meat mixture of two percentages(from 0%to 100%)with lower limit of determination of 1%.This method was used to analyze the actual samples purchased from the market.Results showed that this method is a reliable and effective strategy for meat identification,which could be used for the quantitative detection of adulteration in raw and cooked meat.
作者 胡文彦 徐道坤 刘真 杨军 李春保 冯超彦 HU Wen-yan;XU Dao-kun;LIU Zhen;YANG Jun;LI Chun-bao;FENG Chao-yan(Nanjing Institute for Food and Drug Control,Nanjing 211198,China;College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
机构地区 南京市食品药品监督检验院 南京农业大学食品科学与技术学院
出处 《分析测试学报》 CAS CSCD 北大核心 2020年第12期1481-1486,共6页 Journal of Instrumental Analysis
基金 江苏省重点研发计划项目(BE2016624)。
关键词 多肽标记物 质谱分析 定量检测 肉制品 掺伪鉴别 peptide marker mass spectrometry quantitative testing meat products adulteration identification
分类号 O657.7 [理学—分析化学] TS201.6 [轻工技术与工程—食品科学]
  • 相关文献

参考文献2

二级参考文献14

  • 1Addona TA, Abbatiello SE, Schilling B, Skates SJ, Mani DR, Bunk DM, Spiegelman CH, Zimmerman LJ, Ham AJL, Keshishian H, Hall SC, Allen S, Blackman RK, Borchers CH, Buck C, Cardasis HL, Cusack MP, Dodder NG, Gibson BW, Held JM, Hiltke T, Jackson A, Johansen EB, Kinsinger CR, Li J, Mesri M, Neubert TA, Niles RK, Pulsipher TC, Ransohoff D, Rodriguez H, Rudnick PA, Smith D, Tabb DL, Tegeler TJ, Variyath AM, Vega-Montoto LJ, Wahlander A, Waldemarson S, Wang M, Whiteaker JR, Zhao L, Anderson NL, Fisher SJ, Liebler DC, Paulovich AG, Regnier FE, Tempst P, Carr SA. Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma. Nat Biotechnol, 2009, 27: 633-685.
  • 2Whiteaker JR, Lin C, Kennedy J, Hou L, Trute M, Sokal I, Yan P, Schoenherr RM, Zhao L, Voytovich UJ, Kelly-Spratt KS, Krasnoselsky A, Gafken PR, Hogan JM, Jones LA, Wang P, Amon L, Chodosh LA, Nelson PS, McIntosh MW, Kemp CJ, Paulovich AG. A targeted proteomics-based pipeline for verification of biomarkers in plasma. Nat Biotech, 2011, 29: 625-634.
  • 3Picotti P, Aebersold R. Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions. Nat Methods, 2012, 9: 555-566.
  • 4Huttenhain R, Soste M, Selevsek N, Rost H, Sethi A, Carapito C, Farrah T, Deutsch EW, Kusebauch U, Moritz RL, Nimeus-Malmstrom E, Rinner O, Aebersold R. Reproducible quantification of cancer-associated proteins in body fluids using targeted proteomics. Sci Transl Med, 2012, 4: 1-13.
  • 5Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Nat Methods, 2014, 11: 14-55.
  • 6Wang QH, Wen B, Yan GR, Wei JY, Xie LQ, Xu SH, Jiang DH, Wang TY, Lin L, Zi J, Zhang J, Zhou R, Zhao HY, Ren Z, Qu NR, Lou XM, Sun HD, Du CQ, Chen CB, Zhang SY, Tan FJ, Xian YQ, Gao ZB, He MH, Chen LY, Zhao XH, Xu P, Zhu YP, Yin XF, Shen HL, Zhang Y, Jiang J, Zhang CP, Li LW, Chang C, Ma J, Yan GQ, Yao J, Lu HJ, Ying WT, Zhong F, He QY, Liu SQ. Qualitative and quantitative expression status of the human chromosome 20 genes in cancer tissues and the representative cell lines. J Proteome Res, 2013, 12: 151-161.
  • 7Zhang S, Feng LH, Jiang H, Ma WJ, Korpelainen H, Li CY. Biochemical and proteomic analyses reveal that populus cathayana males and females have different metabolic activities under chilling stress. J Proteome Res, 2012, 11: 5815-5826.
  • 8Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T. A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples. J Proteome Res, 2012, 11: 5311-5322.
  • 9Kroksveen AC, Aaseb? E, Vethe H, van Pesch V, Franciotta D, Teunissen CE, Ulvik RJ, Vedeler C, Myhr KM, Barsnes H, Berven FS. Discovery and initial verification of differentially abundant proteins between multiple sclerosis patients and controls using iTRAQ and SID-SRM. J Proteomics 2013, 78: 312-325.
  • 10Zhang H, Li XJ, Martin DB, Aebersold R. Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nat Biotechnol, 2003, 21: 660-666.

共引文献12

同被引文献19

引证文献1

二级引证文献2

分析测试学报
2020年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部