载去铁敏的3D打印支架修复兔桡骨临界尺寸骨缺损实验研究

Experimental study on a 3D printed scaffold loaded with DFO in repairing critical size bone defect

目的探讨载去铁敏(DFO)的3D打印支架修复兔桡骨临界尺寸骨缺损的效果。方法采用3D打印技术制备载DFO的骨修复支架,扫描电镜观测支架表面形貌。体外实验中,细胞增殖实验验证其细胞相容性,qPCR方法验证支架促MC3T3-E1细胞成骨分化性能;体内实验中,选取成年新西兰大白兔24只,雄性15只,雌性9只,兔龄15~18个月,体重2.5~3.0kg。随机分为空白组(单纯骨缺损)、对照组(空白3D打印支架)、载药组(载DFO的3D打印支架),每组8只。构建兔桡骨临界尺寸骨缺损模型后,分别植入相应支架,于术后即刻和12周时X线观察缺损处愈合情况,并在12周时处死实验兔,对肝肾进行HE染色观察组织相容性。结果扫描电镜显示对照组、载药组3D打印支架形态均匀。体外实验中,细胞增殖实验显示支架细胞相容性好,载DFO的3D打印支架可促MC3T3-E1细胞成骨分化。体内实验证实,在12周时载药组骨缺损修复效果良好,且组织相容性好。结论载DFO的3D打印支架具有良好的促临界尺寸骨缺损修复效能,具有潜在的应用前景。

Objective To explore the effect of 3D printed scaffolds loaded with desferrioxamine(DFO)in repairing critical size bone defects of rabbit radius.Methods The bone repair scaffold with DFO was prepared by 3D printing technology,and the microstructure of the scaffold was observed by scanning electron microscope.In vitro,cell proliferation experiment was used to verify the cytocompatibility,and qPCR methods were used to verify the osteogenic differentiation of MC3T3-E1 cells.In vivo,24 adult New Zealand white rabbits were randomly divided into group A(Blank group,simple bone defect),group B(Control group,blank 3D printed scaffolds)and group C(Experimental group,DFO drug-loaded 3D printed scaffolds).There were 8 rabbits in each group(24 cases of radius).The scaffold was implanted after the radius bone defect model was constructed,and the defect healing was observed by X-rays after the operation immediately and at the 12th week.The rabbits were sacrificed at the 12th week to observe histocompatibility by the liver and kidney stained with HE staining.Results Scanning electron microscopy showed that all the scaffolds had the uniform shape without obvious cytology and tissue toxicity in the control group and experimental group.In vitro,DFO-loaded 3D printed scaffolds could promote the osteogenic differentiation of MC3T3-E1 cells.In vivo,DFO-loaded 3D printed scaffolds showed better performance in bone repairment at the 12th week.Conclusion DFO-loaded 3D printing scaffolds have a good performance in promoting critical size bone defect healing,and have a potential application prospect.