三唑磷对翡翠贻贝神经毒性与氧化损伤效应的性别差异研究

Gender differences in neurotoxicity and oxidative damage of triazophos on Perna viridis

文章以具有环境指示功能的翡翠贻贝(Perna viridis)为研究对象,首次研究了三唑磷对其神经毒性和氧化损伤效应的性别差异性。实验测定了35μg·L−1[1/10096 h半致死浓度(LC50)]三唑磷慢性暴露15 d后雌、雄翡翠贻贝鳃组织中乙酰胆碱酯酶(AchE)和几种抗氧化指标的变化特征。结果显示,三唑磷作用15 d后雌性翡翠贻贝鳃组织中AchE活力无明显变化,而雄性个体中AchE活力被显著抑制。几种抗氧化指标对三唑磷暴露也表现出一定的性别差异,其中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力在雌、雄翡翠贻贝鳃组织中均被显著抑制,丙二醛(MDA)均显著升高。谷胱甘肽硫转移酶(GST)活力和还原型谷胱甘肽(GSH)含量变化则表现出明显的性别差异,仅雄性翡翠贻贝GST活力和GSH含量有显著性变化。综上,三唑磷农药对翡翠贻贝鳃组织表现出明显的神经毒性和氧化损伤效应,其中雄性翡翠贻贝对三唑磷暴露的响应更为敏感。

We studied the gender differences of the neurotoxicity and oxidative damage effects of triazophos on Perna viridis that has function of environmental indicator for the first time.The changes of acetylcholinesterase(AchE)and several antioxidant indices in gill tissues of male and female were determined after 15 d of chronic exposure to 35μg·L−1(1/10096 h LC50).The results show that there was no significant change in the AchE activity in female's gill tissue after being treated with triazophos for 15 d,while the AchE activity in male was significantly inhibited.The activities of superoxide dismutase(SOD)and catalase(CAT)in gill tissues of both male and female were significantly inhibited,and the malondialdehyde(MDA)content was significantly increased.The activity of glutathione S-transferase(GST)and the content of reduced glutathione(GSH)showed significant gender differences.Only the GST activity and GSH content in male had significant changes.In conclusion,triazophos shows obvious neurotoxicity and oxidative damage effects on gill tissue of P.viridis,and the male was more sensitive to triazophos exposure.