420例儿童患者淋巴细胞亚群检测结果分析

Analysis of lymphocyte subsets in 420 children patients

目的分析420例儿童患者淋巴细胞亚群在不同疾病中的分布情况,为临床明确淋巴细胞亚群在不同疾病中的诊断价值提供参考。方法采用流式细胞技术检测420例儿童患者淋巴细胞亚群数量,比较不同疾病淋巴细胞亚群分布的差异。结果420例儿童患者中,数量前三位的诊断为传染性单核细胞增多症121例(28.81%)、发热109例(25.59%)和肺炎63例(15.00%);诊断为上呼吸道感染(26例,6.19%)、支气管炎(24例,5.71%)、急性扁桃体炎(21例,5.00%)的数量相对一致;诊断为淋巴结炎(19例,4.52%)、过敏性紫癜(16例,3.81%)、肝功能异常(11例,2.62%)的数量较少;健康查体(10例,2.38%)的患儿数量最少。与健康儿童相比,传染性单核细胞增多症患儿CD19^+、CD4^+、NK、CD4^+/CD8^+比例下降,发热患儿NK、CD4^+/CD8^+比例下降,支气管炎患儿CD19+比例升高,CD4^+/CD8^+比例下降;所有疾病的CD4^+/CD8^+比例均下降,且传染性单核细胞增多症患儿下降最多;差异均有统计学意义(P<0.05)。结论淋巴细胞亚群CD4^+/CD8^+比例倒置最常见于儿童传染性单核细胞增多症患者。

Objective To analyze the distribution of lymphocyte subsets in 420 children with different diseases,and to provide reference for the clinical diagnosis of lymphocyte subsets in different diseases.Methods Flow cytometry was used to detect the number of lymphocyte subsets in 420 children,and the differences in lymphocyte subsets of different diseases were compared.Results.Of the 420 children,the top three were diagnosed as infectious mononucleosis in 121 cases(28.81%),fever in 109 cases(25.59%),and pneumonia in 63 cases(15.00%);the number of cases diagnosed as upper respiratory tract infection was 26(6.19%),bronchitis was 24(5.71%)and acute tonsillitis was 21(5.00%).There were 19 cases of lymphadenitis(4.52%),16 cases of allergic purpura(3.81%),and 11 cases of abnormal liver function(2.62%).The patient number in 10 children(2.38%)with health examination was the lowest.Compared with the healthy children,CD19^+,CD4^+,NK and CD4^+/CD8^+were decreased in children with infectious mononucleosis,NK and CD4^+/CD8+were decreased in children with fever,CD19+and CD4^+/CD8^+were increased in children with bronchitis,and CD4^+/CD8^+was decreased.The proportion of CD4^+/CD8^+decreased in all diseases,and the most decreased in children with infectious mononucleosis.The differences were statistically significant(P<0.05).Conclusion The CD4^+/CD8^+ratio inversion of lymphocyte subsets is the most common in children with infectious mononucleosis.