TRPC6特异性抑制剂SAR7334对宫颈癌细胞生物学行为影响的研究

Effect of TRPC6 inhibitor SAR7334 on biological behavior of cervical cancer

目的探讨TRPC6在宫颈癌组织中的表达及其抑制剂SAR7334对Siha(人宫颈鳞癌细胞)增殖和迁移的影响。方法选取2017年9月至2019年9月在十堰市人民医院确诊为宫颈鳞癌的11例病例作为实验组,对照组取同一患者正常宫颈组织,免疫印迹法和免疫荧光法等检测宫颈组织中TRPC6蛋白表达;使用20、100、1000 nmol/L SAR7334处理Siha细胞,CCK8法和Hoechst染色检测细胞增殖与凋亡;划痕实验和Transwell法验检测细胞迁移。免疫印迹法检测TRPC6通道阻断后Siha细胞自噬程度的改变。结果宫颈癌组织中TRPC6表达上调;与对照组相比,用100 nmol/L SAR7334阻断TRPC6后,Siha细胞的增殖和迁移减少,划痕愈合率降低,凋亡率增加(P<0.05)。TRPC6通道阻断后,Siha细胞自噬减弱。结论 TRPC6在宫颈癌中表达上调,其抑制剂SAR7334可能通过影响细胞自噬抑制宫颈癌Siha细胞的增殖与迁移。

Objective To investigate the expression of TRPC6 in cervical cancer and the effect of its inhibitor SAR7334 on the proliferation and migration of Siha(Human cervical squamous cell carcinoma cell). Methods Eleven cases of cervical squamic carcinoma diagnosed in People’s hospital of Shiyan from September 2017 to September 2019 were selected as the experimental group. Normal cervical tissue of the same patient was used as the control group. Western-blot and immunofluorescence were used to detect the expression of TRPC6;Siha cells were treated with 20, 100, 1000 nmol/L SAR7334, cell proliferation and apoptosis were detected by CCK8 method and Hoechst staining. Cell migration was detected by scratch test and Transwell test. After TRPC6 channel was blocked, the change of autophagy in Siha cells was detected by immunoblotting. Results The expression of TRPC6 protein in cervical cancer tissues was significantly higher than that of normal tissues. Compared with the control group, after blocking TRPC6 with 100 nmol/L SAR7334, the proliferation and migration of Siha cells reduced, the scratch healing rate reduced, and the apoptosis rate increased(P<0.05). After the TRPC6 channel was blocked, the autophagy of Siha cell weakened. Conclusions The expression of TRPC6 is up-regulated in cervical cancer, and its inhibitor SAR7334 may inhibit the proliferation and migration of cervical cancer Siha cells by affecting cell autophagy.