大豆根中GmPME7基因防御氧化铜纳米颗粒的功能验证

Functional Identification of GmPME7 Gene Responding to Copper Oxide Nanoparticles in Soybean Roots

通过构建pCAMBIA3301-GmPME7载体,在大豆毛状根中超表达GmPME7基因,验证了该基因的功能。结果表明:氧化铜纳米颗粒(CuO NPs)处理后,与转入空载体的毛状根相比,转基因毛状根中的果胶甲酯酶(PME)活性逐渐增加,果胶甲酯化程度逐渐降低,2 mg/L CuO NPs处理48 h后的转基因毛状根中PME活性和果胶甲酯化程度分别为对照的1.23倍和0.69倍,说明GmPME7基因的功能可能是通过调控PME活性增加从而降低果胶甲酯化程度,来抵抗CuO NPs的胁迫。

Through the constructed pCAMBIA3301-GmPME7 vector,the GmPME7 gene was overexpressed in soybean hairy roots,and the function of GmPME7 gene was verified.Compared with the control of hairy roots with empty vector of the pCAMBIA3301,the PME activity gradually increased and the degree of pectin methylation gradually decreased in overexpressing roots under copper oxide nanoparticles(CuO NPs)suspensions treatment.After treatment with 2 mg/L CuO NPs suspension for 48 h,the PME activity and the degree of pectin methylation in overexpressor lines were 1.23 and 0.69 times,compared to control group.It illustrated that the GmPME7 gene probably resisted the stress of CuO NPs through regulating the PME activity and the degree of pectin methylation in soybean root.