杏香兔耳风中1个新的愈创木烷型倍半萜四糖苷

A new guaiane sesquiterpene glycoside from Ainsliaea fragrans

目的研究杏香兔耳风Ainsliaeafragrans全草的化学成分。方法采用硅胶、葡聚糖凝胶LH-20等柱色谱方法以及制备液相色谱技术进行分离纯化,并综合运用核磁共振波谱和质谱鉴定化合物结构。结果从杏香兔耳风全草乙醇提取物中分离得到了9个化合物,分别鉴定为中美菊素C-3-O-β-葡萄糖-(1→3)-β-葡萄糖-(1→3)-β-葡萄糖-(1→3)-β-葡萄糖苷(1)、macrocliniside B(2)、macrocliniside I(3)、异绿原酸C(4)、4,5-O-二咖啡酰基奎宁酸甲酯(5)、异绿原酸A(6)、3,5-O-二咖啡酰基奎宁酸甲酯(7)、3,4-O-二咖啡酰基奎宁酸甲酯(8)、紫云英苷(9)。结论化合物1为1个新的愈创木烷型倍半萜四糖苷,命名为杏香兔耳风苷A(ainslifragside A)。化合物2、3、5、7~8为首次报道从该植物中分离得到。

Objective To investigate the chemical constituents in whole plant of Ainsliaea fragrans.Methods Silica gel,Sephadex LH-20 gel column chromatography,and semi-preparative HPLC were used to separate and purify the chemical constituents of ethanol extract.And the structures were elucidated on the basis of NMR and MS spectroscopic data.Results Nine compounds were isolated and identified as zaluzanin C-3-O-β-glucopyranosyl-(1→3)-β-glucopyranosyl-(1→3)-β-glucopyranosyl-(1→3)-β-glucopyranoside(1),macrocliniside B(2),macrocliniside I(3),isochlorogenic acid C(4),4,5-O-di-caffeoylquinic acid methyl ester(5),isochlorogenic acid A(6),3,5-O-di-caffeoylquinic acid methyl ester(7),3,4-O-di-caffeoylquinic acid methyl ester(8),and astragalin(9).Conclusion Compound 1 is a new guaiane sesquiterpene glycoside named as ainslifragside A.Compounds 2,3,5,7,and 8 are isolated from this plant for the first time.