HLA-G基因多态性与2型糖尿病遗传易感性的相关性研究

Study on association between HLA-G gene polymorphism and genetic predisposition of type 2 diabetes

目的分析HLA-G基因多态性与2型糖尿病遗传易感性的相关性。方法选取2016年1月至2017年10月在湖北医药学院附属东风医院内分泌科住院的2型糖尿病患者103例为研究对象,健康体检志愿者321例为对照。提取全血基因组DNA,采用聚合酶链反应(PCR)和Sanger测序法检测HLA-G rs12722477位点的基因多态性。结果HLA-G基因rs12722477位点3种基因型的分布均符合Hardy-Weinberg平衡(χ^2=5.198,P=0.074)。HLA-G基因rs12722477位点的A等位基因在糖尿病组的频率略高于对照组,但差异无统计学意义(P=0.054),AA基因型在糖尿病组中出现的频率明显高于对照组(P=0.010)。AA基因型的淋巴细胞计数高于CC基因型和CA基因型(P=0.006、0.003);AA基因型与CC基因型的糖化血红蛋白水平比较,差异有统计学意义(P=0.029)。logistic回归分析显示,HLA-G基因rs12722477位点AA基因型在隐性模型中增加糖尿病的患病风险(OR=3.954,95%CI:1.132~13.811,P=0.031)。结论HLA-G基因rs12722477位点多态性与糖尿病的发生有关,AA基因型增加了糖尿病的患病风险,A等位基因为糖尿病的风险基因。

Objective To evaluate the association between HLA-G gene polymorphism and genetic predisposition of type 2 diabetes.Methods A total of 103 patients with type 2 diabetes who were hospitalized in the hospital from January 2016 to October 2017 were selected as study subjects,and 321 healthy physical examination volunteers were selected as the control group.Whole blood genomic DNA was extracted.The polymorphism of HLA-G rs12722477 stie was detected by polymerase chain reaction(PCR)and Sanger sequencing.Results The polymorphisms′distribution of HLA-G gene rs12722477 stie followed Hardy-Weinberg equilibrium(χ^2=5.198,P=0.074).For the HLA-G gene rs12722477 stie,the frequency of A allele in the diabetic group was longer than that of the control group,but the difference was not statistically significant(P=0.054).The AA genotype appeared more frequently in the diabetic group(P=0.010).The lymphocyte count with AA genotype was significantly higher than that with CC and CA genotype(P=0.006,0.003).The glycosylated hemoglobin level in AA genotype was significantly higher than that in CC genotype(P=0.029).Logistic regression analysis showed that the HLA-G gene rs12722477 stie AA genotype increased the risk of diabetes in recessive model(OR=3.954,95%CI:1.132-13.811,P=0.031).Conclusion The rs12722477 stie polymorphism of HLA-G gene was related to the occurrence of diabetes.AA genotype increased the risk of diabetes,and A allele was a risk gene for diabetes.