全自动血液分析仪在有核细胞分析中的价值研究

Value of fully automatic hematology analyzer in nuclear cell analysis

目的探讨全自动血液分析仪在有核细胞分析中的价值。方法收集2019年1-4月该院血细胞标本220份,给予全自动血液分析仪及人工镜检,比较检测时间、有核细胞计数、不成熟及异常细胞情况。结果全自动血液分析仪检测时间平均(3.56±1.02)min,明显少于人工镜检(8.02±1.00)min,差异有统计学意义(P<0.05)。全自动血液分析仪检测中性粒细胞、淋巴细胞、单核细胞和嗜酸性粒细胞计数与人工镜检比较差异无统计学意义(P>0.05)。全自动血液分析仪在幼稚粒细胞、红细胞形态异常、异型淋巴细胞、杆状粒细胞及原始细胞检测中存在假阳性,符合率分别为25.00%、11.11%、20.00%、25.00%和50.00%。结论全自动血液分析仪在有核细胞分析中有一定应用价值,但在不成熟及异常细胞检测中,其与人工镜检符合率较低。

Objective To explore the value of fully automatic hematology analyzer in nuclear cell analysis.Methods A total of 220 blood cell samples from January to April 2019 were collected,all samples were examined by fully automatic hematology analyzer and artificial microscopy.The detection time,nuclear cell count,immature and abnormal cells were compared.Results The detection time of sample by fully automatic hematology analyzer was(3.56±1.02)min,which was significantly less than that by artificial microscopy(8.02±1.00)min,and the difference was statistically significant(P<0.05).There was no significant difference in the count of neutrophils,lymphocytes,monocytes and eosinophils measured by automatic hematology analyzer compared with that by manual microscopy(P>0.05).There were false positives in the detection of juvenile granulocytes,erythrocyte abnormalities,atypical lymphocytes,rod granulocytes and primordial cells by fully automatic hematology analyzer,the coincidence rate was 25.00%,11.11%,20.00%,25.00%and 50.00%,respectively.Conclusion The automatic hematology analyzer has certain application value in nuclear cell analysis.However,the coincidence rate with artificial microscopy is relatively low in immature and abnormal cells.