内标法和外标法用于测定人血中多西他赛药物浓度的比较分析

Internal Standard Method and External Standard Method are Used to Determine the Concentration of Docetaxel in Human Blood Comparative Analysis

目的:比较内标法和外标法用于测定人血中多西他赛药物浓度的差异。方法:分别以内标法和外标法建立人血中多西他赛药物浓度测定的超高效液相测定方法,并进行方法学验证,最后测定不同的血液样本,比较两种方法的测定结果。结果:以内标法和外标法为基础的多西他赛超高效液相测定方法均通过方法学验证,对于人血中多西他赛的含量测定,内标法和外标法无显著性差异。结论:外标法操作简便,结果准确,适用于人血中多西他赛的含量测定。

Objective: To compare the internal standard method and the external standard method used to determine the difference in the concentration of docetaxel in human blood. Methods: The internal standard method and the external standard method were used to establish the ultra-high performance liquid method for the determination of the concentration of docetaxel in human blood, and the method was verified. Finally, different blood samples were measured and the results of the two methods were compared. Results: Both the internal standard method and the external standard method based on the ultra-high performance liquid method for the determination of docetaxel passed the methodological verification. For the determination of the content of docetaxel in human blood, the internal standard method and the external standard method are not significant difference. Conclusion: The external standard method is simple and accurate, and it is suitable for the determination of docetaxel in human blood.