摘要
本文首先将pH特异性识别电化学探针(NB)和电化学内参比分子(ABTS)分子通过π-π作用共修饰于单壁碳纳米管(CNT)修饰的碳纤维电极(CFME)表面。结果发现,该电极对pH检测线性范围为4.52~8.83,对抗坏血酸(AA)检测具有较宽的线性范围(0.02~8.0 mM)。与经典pH玻璃电极测定方法对比,采用该微传感器重现性好、稳定性高、准确度高。最后碳纤维电极的小尺寸进一步实现一滴全血样品pH和AA的同时检测取得满意的结果。
pH specific recognition electrochemical probe(NB)and electrochemical internal reference molecule(ABTS)are co-modified on the surface of carbon nanotube(CNT)modified carbon fiber electrode(CFME)throughπ-πinteraction.The results showed that the electrode has a linear range of 4.52~8.83 for pH detection and a wide linear range(0.02~8.0 mM)for AA.Compared with the classic pH glass electrode,the micro-sensor has good reproducibility,high stability and high accuracy.Finally,the small size of the carbon fiber electrode was further the simultaneous detection of pH and AA of a drop of whole blood sample with satisfactory results.
作者
赵乐
何雨蒙
纪庆敏
王亚宁
周艳丽
韦秀华
董辉
Zhao Le;He Yumeng;Ji Qingmin;Wang Ya’ning;Zhou Yanli;Wei Xiuhua;Dong Hui(College of Biology and Food,Shangqiu Normal University,Shangqiu 476000;Henan Key Laboratory of Biomolecular Recognition and Sensing,College of Chemistry and Chemical Engineering,Shangqiu Normal University,Shangqiu 476000,China)
出处
《广东化工》
CAS
2020年第22期242-244,共3页
Guangdong Chemical Industry
基金
2021年度河南省高等学校重点科研项目资助计划(项目编号:21A150043)。
关键词
PH
抗坏血酸
电化学微传感器
全血
pH
ascorbic acid
electrochemical microsensor
whole blood
