张家界大叶黄杨炭疽病的病原菌鉴定

Pathogen identification of anthracnose on Euonymus japonicus in Hunan Province

对从湖南省张家界市永定区采集的疑似炭疽病的大叶黄杨病叶进行分离培养,获得1类纯化菌株,挑选代表菌株进行致病性测定,通过柯赫氏法则确定其为致病菌。依据菌落特征、分生孢子的形态特征,结合多基因的系统发育分析,代表分离菌株与暹罗炭疽菌(Colletotrichum siamense)聚为一支。

The diseased leaves of Euonymus japonicus collected from Yongding District,Zhangjiajie City,Hunan Province were collected,and pathogen isolation and cultivation were conducted.A purified class 1 strain was obtained,and the representative strains were selected for pathogenicity test and identified as pathogenic fungi by Koch’s law.According to the characteristics of colony and conidia morphology,combined with the phylogenetic analysis of polygenes,the representative isolates were clustered with Colletotrichum siamense to one branch.