微等离子体射频技术对窄波中波紫外线诱导豚鼠皮肤色素沉着的影响

The use of micro-plasma radio frequency technology to treat narrow band ultraviolet B induced guinea pigs skin pigmentation

目的探讨微等离子体射频技术治疗窄波中波紫外线(narrow band ultraviolet B,NB-UVB)诱导下豚鼠皮肤色素沉着动物模型的效果。方法选择普通级健康豚鼠10只,对豚鼠棕黄色毛发皮肤区域脱毛后行NB-UVB照射,建立UVB诱导下豚鼠皮肤色素沉着模型。每只豚鼠造模成功区域随机分为4组,A组为空白对照组,不进行治疗;B、C、D组均为微等离子体射频治疗组,以不同能量参数的微等离子体射频技术进行治疗,B组输出功率10 W,每个发射点治疗能量0.06 J;C组输出功率20 W,治疗能量0.12 J;D组输出功率30 W,治疗能量0.18 J。分别在治疗前和治疗后2、4、6周,取材进行黑色素细胞染色(表皮分离-多巴染色法),观察酪氨酸酶活性的黑色素细胞数目,黑素颗粒染色(Masson-Fontana法)观察黑素颗粒面积占表皮面积百分比,免疫组织化学染色(c-kit蛋白)观察黑色素细胞活性的改变。应用SPSS 13.0统计软件对实验数据进行统计学分析,P<0.05为差异有统计学意义。结果治疗结束后2、4、6周,微等离子体射频治疗的B、C、D组酪氨酸酶活性的黑色素细胞数目较A组低,差异有统计学意义(P<0.05),且黑色素细胞数目减少与微等离子体射频治疗能量增加呈正相关。治疗结束后2、4周,微等离子体射频技术治疗的B、C、D组色素沉着皮肤表皮黑素颗粒较A组低,治疗结束后6周,仅D组低于A组,差异有统计学意义(P<0.05),并且皮肤表皮黑素颗粒减少与微等离子体射频能量增加呈正相关。实验设置的微等离子体射频治疗组能量未引起c-kit阳性细胞数目增加,治疗结束后2周各组间c-kit阳性细胞数目,A组较B、C、D组高,差异有统计学意义(P<0.05);B、C、D组间比较差异无统计学意义(P>0.05);微等离子体射频照射后4、6周A、B、C、D组均未见c-kit阳性细胞。结论微等离子体射频可加速豚鼠皮肤色素沉着的消退,对改善NB-UVB照射后豚鼠皮肤色素沉着的动物模型有效。

Objective To study on the effects of micro-plasma radio frequency technology on guinea pig skin pigmentation animal model induced by narrow band ultraviolet B(NB-UVB).Methods We selected ten healthy guinea pigs(common grade),removed the brown hair of the guinea pig and established UVB to induce the skin pigmentation model of guinea pig.The pigmentation zone was randomly divided into four groups:Group A was used as blank control with no treatment and Groups B,C and D were treated with micro-plasma radio frequency technology using different energy parameters.Group B treatment parameters were as follows:output power was 10 watts,and treatment energy was 0.06J.Group C treatment parameters were as follows:output power was 20 watts,and treatment energy was 0.12J.Group D treatment parameters were as follows:output power was 30 watts,and treatment energy was 0.18J.They were compared before treatment and at 2,4,and 6 weeks after treatment,based on melanin cell staining(epidermis separation-dopa staining)and melanin granules dyeing method(Masson-Fontana).Immunohistochemical staining(c-kit protein)was used to observe the tyrosinase activity in the melanocytes,melanin granule percentage area of the skin area,and the change in melanin cell activity.SPSS13.0 statistical software was used for statistical analysis of the experimental data.The significance of the difference(P<0.05)was measured.Results The results showed that micro-plasma radio frequency(RF)technology treatment could reduce the tyrosinase activity in the melanocytes,2,4 and 6 weeks after the end of treatment:Groups B,C and D were lower than group A,and the difference was statistically significant(P<0.05),and as the treatment energy increased,the speed of reduction was increased.Micro-plasma radio frequency technology could reduce skin pigmentation and decrease the amount of epidermal melanin granules,2 and 4 weeks after the end of treatment:Groups B,C and D were lower than Group A;6 weeks after the end of treatment,only Group D was lower than Group A,with statistically significant differences(P<0.05).An increase in the energy of the micro-plasma radio frequency sped up the decline in the amount of epidermal melanin granules.The number of c-kit positive cells was not increased in this experiment.Two weeks after the end of treatment,the number of c-kit positive cells was higher than those of Groups B,C and D.There were no statistically significant differences in Groups B,C and D(P<0.05).No c-kit positive cells were found in Groups A,B,C and D at 4 and 6 weeks after the irradiation.Conclusions Micro-plasma radio frequency technology could accelerate the fading of guinea pig skin pigmentation.It is effective to improve pigmentation of guinea pig skin after NB-UVB irradiation.