摘要
Objective The aim of the present study was to investigate the hypoglycemic effects of silkworm extract(SE)on experimental type 2 diabetes mellitus(T2DM)rats.Methods SE was prepared by dissolving freeze-dried silkworm powder in 70%(v/v)aqueous ethanol.T2DM rats were induced by feeding them a high fat diet and an intraperitoneal injection of streptozotocin(STZ).The blood glucose,free fatty acid(FFA),malondialdehyde(MDA),tumor necrosis factor alpha(TNF-α)and superoxide dismutase(SOD)levels were detected by enzyme-linked immunosorbent assay(ELISA).The quality of SE was controlled by high performance liquid chromatography(HPLC;Agilent 1260,Agilent,USA).Hematoxylin-eosin(HE)staining was performed for histological evaluation.Antibody expression was assessed via immunohistochemistry(IHC)staining.Results SE could improve insulin resistance and islet cell function by reducing FFA,MDA and TNF-αlevels and increasing SOD level.In addition,pancreatic HE staining analysis revealed that SE has a protective effect on isletβ-cells.Conclusions The present study indicates that SE has hypoglycemic as well as pancreatic protective effects in T2DM model rats.
目的本研究旨在观察家蚕提取物(SE)对2型糖尿病(T2DM)大鼠的降血糖作用。方法将冻干的全蚕粉溶于70%(v/v)的乙醇水溶液中制备SE。通过喂养高脂饮食和腹腔注射链脲佐菌素(STZ)来建立T2DM大鼠模型。采用酶联免疫吸附法(ELISA)检测血糖,游离脂肪酸(FFA),丙二醛(MDA),肿瘤坏死因子α(TNF-α)和超氧化物歧化酶(SOD)水平。利用高效液相色谱法(HPLC)对SE进行质量控制。通过胰腺组织的苏木精-伊红(HE)染色和免疫组化(IHC)染色进行组织学评估以及抗体表达监测。结果SE可通过降低FFA,MDA和TNF-α水平以及增加SOD水平改善胰岛素抵抗和胰岛细胞功能。另外,胰腺HE染色和IHC染色结果表明,SE对胰岛β细胞具有保护作用。结论本研究表明SE对T2DM模型大鼠具有降血糖和胰腺保护作用。
基金
supported by the Xiamen City Health and Family Planning Commission and the Xiamen Municipal Bureau of Science and Technology
the funding support from the Leading Project of Fujian. P. R. C. (No. 2019D018)
the Xiamen Science and Technology Project (No. 3502Z20174058)。
