ICP-MS测定茖葱中37种微量元素

ICP-MS determination of 37 trace elements in Allium victorialis L.

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)法测定药用植物茖葱茎叶中微量元素的含量。方法本研究采用HNO3-H2O2体系,将茖葱茎叶粉碎(过200目筛),湿法消解后,以In、Rh为内标元素,用ICP-MS法测定其微量元素的含量。结果37种微量元素质量浓度范围为0.008243~886.2μg·L^-1,回归方程相关系数r均≥0.9996,检测限为0.0002~0.1240μg·L^-1,加样回收率为97.11%~100.30%,RSD≤5.0%。茖葱茎叶中富含微量元素,其中Fe元素的含量最高,其次为Mn、Sr、Ba、Zn、B元素,重金属元素Cu、As、Pb、Cd的含量均低于国家标准限量值。结论该方法检测限低,准确度高,精密度好,可快速对茖葱茎叶中的37种微量元素同时进行测定,为茖葱的进一步研究及应用提供了理论依据。

Objective To determine the trace elements in Allium victorialis L.by inductively coupled plasma spectrometry(ICP-MS)with microwave digestion.Methods The stem and leaf of Allium victorialis L.were used as samples.The contents of trace elements were determined by ICP-MS after the samples were diluted with wet digestion in HNO3-H2O2 system.In and Rh were used as the internal standard.Results The concentration of 37 trace elements was 0.008243~886.2μg·L^-1.The correlation coefficient r of each element was greater than 0.9996,and the detection limit was 0.0002~0.1240μg·L^-1.The recovery rates were between 97.11%~100.03%,with RSD no greater than 5.0%.The stem and leaf of Allium victorialis L.were rich in a variety of elements.The content of Fe was the highest,followed by Mn,Sr,Ba,Zn,and B elements.The contents of Cu,As,Pb and Cd.were lower than those of national standard.Conclusion The established rapid determination method has good sensitivity,high accuracy,and good precision,and can be used for the quick determination of 37 trace elements,providing a theoretical basis for further research and application of Allium victorialis L..