二氧化钛纳米管负载京尼平交联的壳聚糖水凝胶对大鼠骨髓间充质干细胞活性的影响

Effect of titanium dioxide nanotubes loaded with genipin crosslinked chitosan hydrogel on biological activity of mouse bone marrow mesenchymal stem cells

目的:探讨电化学阳极氧化形成的二氧化钛纳米管负载壳聚糖水凝胶,京尼平交联后对大鼠骨髓间充质干细胞(BMSC)体外生物活性的影响。方法:利用两步法电化学阳极氧化技术在纯钛表面制备规则的二氧化钛纳米管阵列,负压法负载壳聚糖涂层,京尼平交联。用场发射扫描电镜观察各组样品形貌,接触角测量仪测亲水性。样品与BMSC共培养,用CCK-8法检测细胞粘附和增殖情况。结果:阳极氧化法+负压法构建复合微纳米结构,场发射扫描电镜检测样品见表面粒状颗粒物,亲水性增加,细胞实验结果证实水凝胶-金属复合物对BMSC的粘附无明显影响,可促进细胞增殖。结论:二氧化钛纳米管负载京尼平交联的壳聚糖水凝胶复合材料可提高BMSC细胞活性。

Objective:To investigate the effect of titanium nanotubes formed by electrochemical anodization,loaded with genipin crosslinked chitosan hydrogel on biological activity of mouse bone marrow mesenchymal stem cells(BMSC)in vitro.Methods:A two-step electrochemical anodization technique was used to prepare a regular array of titanium dioxide nanotubes on the substrate of titanium,chitosan coating was loaded by negative pressure method,and crosslinked with genipin.Field emission scanning electron microscope was used to observe the morphology of different substrates,and the contact angle measuring instrument was used to measure the hydrophilicity.The samples were co-cultured with BMSC,and cell adhesion and proliferation were measured by the CCK-8.Results:Anodic oxidation method and negative pressure method was used to construct composite nano-structures.Field emission scanning electron microscopy showed particles on the surface,which showed increased hydrophilicity.The results of cell experiments confirmed that the nanocomposite had no obvious effect on the adhesion of BMSC,but promoted cell proliferation.Conclusions:Nanocomposites of titanium dioxide nanotubes loaded with genipin crosslinked chitosan hydrogel can improve BMSC activity.