淫羊藿苷通过调节Nrf2/HO-1信号通路减轻大鼠肾缺血再灌注损伤

Icariin attenuates renal ischemia-reperfusion injury in rats by regulating Nrf2/HO-1 signaling pathway

目的:研究淫羊藿苷(ICA)对大鼠肾缺血再灌注损伤(RIRI)的作用及分子机制。方法:选取40只SD大鼠构建RIRI模型,随机分为5组:假手术组、ICA组(60 mg/kg)、RIRI组、RIRI+ICA低剂量组(30 mg/kg)和RIRI+ICA高剂量组(60 mg/kg),每组8只。HE染色检测肾组织病理损伤;检测血清中尿素氮(BUN)、肌酐(Cr)水平以评估肾功能;ELISA检测血清中TNF-α、IL-1β和IL-6水平;检测氧化应激水平(SOD和MDA水平);TUNEL检测细胞凋亡情况;Western blot检测相关蛋白表达水平。结果:ICA剂量依赖性地改善缺血再灌注引起的肾组织损伤和肾功能紊乱,减轻炎症反应和氧化应激,抑制细胞凋亡。进一步机制分析表明,ICA通过激活Nrf2/HO-1/NQO1信号通路改善大鼠RIRI。结论:ICA剂量依赖性地通过激活Nrf2/HO-1/NQO1信号通路改善缺血再灌注引起的大鼠肾损伤。

Objective:To study effect and molecular mechanism of icariin(ICA)on renal ischemia-reperfusion injury(RIRI)in rats.Methods:40 SD rats were selected to construct RIRI models,and were randomly divided into 5 groups:sham operation group,ICA group(60 mg/kg),RIRI group,RIRI+ICA low close(30 mg/kg)group and RIRI+ICA high close(60 mg/kg)group,with 8 rats in each group.HE staining was used to detect pathological damage of renal tissue.Serum blood urea nitrogen(BUN)and creatinine(Cr)levels were measured to evaluate renal function.ELISA was employed to detect levels of TNF-α,IL-1βand IL-6 in serum.Detected oxidative stress level(SOD and MDA levels).TUNEL was utilized to detect apoptosis.Western blot was applied to detect expression levels of related proteins.Results:ICA dose-dependently improved renal tissue damage and renal dysfunction caused by ischemia-reperfusion,reduced inflammation and oxidative stress,and inhibited apoptosis.Further mechanism analysis showed that ICA improved rats RIRI by activating Nrf2/HO-1/NQO1 signaling pathway.Conclusion:ICA improved rats renal injury induced by ischemia-reperfusion by activating Nrf2/HO-1/NQO1 signaling pathway in a dose-dependent manner.