SAA水平及其基因多态性分析在川崎病中的临床研究价值

The clinical value of SAA level and its polymorphism analysis in Kawasaki disease

目的探讨血清淀粉样蛋白A(SAA)水平在川崎病(KD)患儿中的变化及临床意义,SAA1rs12218、SAA2rs2468844基因多态性与KD易感性之间的关系。方法选取90例KD患儿作为KD组,选取同期100例健康儿童作为对照组,利用散射免疫比浊法检测SAA水平;采用基于聚合酶链式反应(PCR)的序列分型方法对两种单核苷酸多态性SAA1rs12218和SAA2rs2468844进行分析。结果KD组SAA水平较对照组明显升高,经治疗后SAA水平明显下降,差异有统计学意义(P<0.05);SAA1rs12218、SAA2rs2468844位点TT、TC、CC基因型分布和T、C等位基因分布在KD组与对照组、KD-CAL组与KD-NCAL组之间差异均无统计学意义(P>0.05)。结论SAA水平可能有助于监测KD病程;尚未发现SAA1rs12218、SAA2rs2468844位点多态性与KD及其冠脉损伤的发生存在关联性。

Objective To investigate the changes and clinical significance of serum amyloid A(SAA)level in children with Kawasaki disease(KD),and to investigate the association between the polymorphisms of SAA1rs12218 and SAA2rs2468844 gene and KD susceptibility.Methods A total of 90 KD children were selected as KD group,and 100 healthy children were taken as control group,the level of SAA was measured by scatter immunoturbidimetry.Two single nucleotide polymorphisms SAA1rs12218 and SAA2rs2468844 were deteted by polymerase chain reaction sequence-based typing(PCR).Results SAA level in KD group significantly increased compared with that in control group,and SAA level significantly decreased after treatment,and the differences were statistically significant(P<0.05).There was no statistical difference in the distribution of the locus SAA1rs12218 and SAA2rs2468844 genotype TT,TC and CC and allele T and C between KD group and control group,and between KD-CAL group and KD-NCAL group(P>0.05).Conclusion SAA level may help to monitor KD course.No association was found between loci SAA1rs12218 and SAA2rs2468844 polymorphism of SAA gene and the risk of KD or its complication of coronary artery lesion.