摘要
为了进一步了解寒冷环境下民猪抗寒的分子机制,本试验采用Real-time PCR方法检测寒冷环境下PDK4 mRNA在民猪背最长肌中的表达变化情况。结果表明:寒冷环境下36 h和21 d民猪背最长肌中PDK4 mRNA表达量均显著高于常温组。
To study the molecular mechanism of cold resistance of Min pig under cold environment,the real-time PCR was applied to analyze of PDK4 in Min longissimus dorsi muscles(LM)under cold environment.Under cold conditions 36 h and 21 d PDK4 expression were increased significantly than that of normal group.
作者
李忠秋
刘春龙
吴赛辉
张冬杰
孙金艳
彭福刚
王文涛
刘娣
LI Zhong-qiu;LIU Chun-long;ZHANG Dong-jie;SUN Jin-yan;PENG Fu-gang;MA Hong;WANG Wen-tao;LIU Di(Animal Husbandry Research Centre of Heilongjiang Academy of Agricultural Sciences,Key Laboratory of Combining Farming and Animal Husbandry,Ministry of Agriculture and Rural Affairs,P.R.China,Harbin 150086,China;Northeast Institute of Geography and Agricultural Ecology,Chinese Academy of Sciences,Key Laboratory of Black Soil Ecology, Harbin 150081,China)
出处
《黑龙江农业科学》
2020年第12期61-63,共3页
Heilongjiang Agricultural Sciences
基金
黑龙江省农业科学院院级课题(2018KYJL005)
黑龙江省自然科学基金(LH2019C064)
现代农业产业技术体系建设专项(CARS-36)。
