明胶辅助共沉淀法合成硫化铜纳米材料及其光热消融癌细胞

Gelatin-mediated coprecipitation synthesis of copper sulfide nanoparticles for photothermal ablation of cancer cells

以明胶(Gelatin)为配体,通过共沉淀法合成CuS-gelatin纳米材料。研究明胶用量、反应温度和时间对CuS纳米材料的形貌、晶相、光吸收等方面的影响。遴选出最优材料,探索其光热转换性能、细胞毒性和癌细胞光热消融效果。结果表明:最佳明胶用量为0.25~0.5 g,提高温度或增加时间会使CuS样品的尺寸增大且结晶度提高。当明胶用量为0.5 g,80℃下反应6 h得到的CuS-gelatin纳米颗粒具有最强的近红外光吸收能力。用功率密度为1.0 W/cm 2的1064 nm激光照射0.1 mL浓度为0.4 mg/mL的CuS-gelatin纳米颗粒分散液,在300 s内CuS-gelatin分散液升温约30.8℃,光热转换效率达到35.7%。与纯CuS相比,CuS-gelatin表现出较低的细胞毒性,在1064 nm激光照射10 min后其能有效地光热消融癌细胞。

CuS nanomaterials were prepared through a gelatin-mediated co-precipitation method.The effects of gelatin amount,reaction temperature and time on the morphology,phase and photo absorption were investigated.The results show that the optimized gelatin amount is 0.25~0.5 g,and the increased temperature or time can enhance the size and crystalline of CuS nanomaterials.CuS nanoparticles prepared with 0.5 g gelatin at 80℃for 6 h have the strongest near-infrared(NIR)photoabsorption.The 0.1 mL aqueous dispersion containing 0.4 mg/mL CuS-gelatin which is continuously irradiated by the 1064 nm laser with a power density of 1.0 W/cm 2,and the temperature rising reaches a maximum value of 30.8℃at 300 s,yielding a photothermal conversion efficiency of 35.7%.In addition,compared with bare CuS nanoparticles,CuS-gelatin exhibits the lower cytotoxicity.Importantly,cancer cells in vitro can be efficiently ablated by the photothermal effect of CuS-gelatin with the irradiation of 1064 nm laser for 10 min.