富血小板血浆对兔骨关节炎软骨中基质金属蛋白酶-13表达的影响

Inhibition effect of platelet-rich plasma on matrix metalloproteinase-13 expression in rabbit osteoarthritis cartilage

目的探讨富血小板血浆(platelet-rich plasm,PRP)抑制兔骨关节炎软骨表达基质金属蛋白酶-13(matrix metalloproteinase-13,MMP-13)的表达而发挥软骨保护作用。方法选取30只成年清洁级新西兰大白兔,采用数字表法随机分为对照组、关节炎组、PRP组,每组各10只。关节炎组和PRP组按Hulth法制备后肢左膝关节炎模型,对照组行假手术。造模后第1周开始,各组抽取耳缘静脉血制备PRP 0.2 mL,仅PRP组给予关节腔注射。至第6周处死实验动物,获取各组左膝关节,分别行软骨组织HE染色测量软骨厚度,免疫组织化学染色计数MMP-13阳性细胞数,免疫荧光及Western blotting法检测MMP-13表达量。结果对照组显示关节软骨形态接近正常,关节炎组软骨破坏明显,而PRP干预组较关节炎组,关节厚度得以保留,MMP-13阳性细胞及其表达量明显受到抑制。结论PRP可减少MMP-13软骨细胞的数量和抑制MMP-13表达而发挥软骨保护作用。

Objective To investigate the protective effect of platelet-rich plasm(PRP)to inhibit expression of matrix metalloproteinase-13(MMP-13)in rabbit knee osteoarthritis.Methods Thirty New Zealand adult clean class rabbits were randomly divided into three groups,the blank control group,osteoarthritic group,and PRP group,ten rabbits in every group.The latter two groups underwent surgery to establish knee osteoarthritis(OA)model with Hulth method,and the control group underwent sham operation.One week after operation,0.2 mL PRP was extracted from venous blood of ear margin in every group.Only the PRP group received articular injection.All experimental animals were killed at 6 th week,and the left knee joints were obtained.Cartilage thickness was measured under a microscope with HE staining,the number of MMP-13 positive cells was counted with immunohistochemical staining,and the expression of MMP-13 was detected with immunofluorescence and Western blotting.Results The control group showed the morphology of articular cartilage close to normal.The cartilage damage was obvious in the arthritis group.In the PRP intervention group,the joint cartilage thickness was preserved,and the MMP-13 positive cells and their expression were significantly inhibited.Conclusion PRP can protect cartilage by reducing the number of MMP-13 chondrocytes and inhibiting the expression of MMP-13.