17个建兰品种光合特性分析

Analysis of Photosynthetic Characteristics of 17 Varieties of Cymbidium ensifolium

以17个建兰(Cymbidium ensifolium)品种为材料,采用改良的丙酮法提取叶绿素,再通过Arnon丙酮法公式计算光合色素含量,利用捷克FluorCam开放式叶绿素荧光仪测定不同品种的叶绿素荧光参数。结果表明,17个建兰品种的光合色素和叶绿素荧光参数具有不同程度的差异,其中‘铁骨素’(C.ensifolium‘Tiegusu’)、‘逸红双娇’(C.ensifolium‘Yihongshuangjiao’)和‘闽南黄蝶’(C.ensifolium‘Minnanhuangdie’)的光合色素含量高于其他品种,表明这3个品种具有良好的光合效率,吸收光能的能力较强;‘铁骨素’最大荧光产量(Fm)、Kautsky诱导效应最大荧光(Fp)、PSⅡ原初光能转化效率(Fv/Fm)和非光化荧光淬灭系数(NPQ)均为最高。综上可知,‘铁骨素’的光合生理特性优于其他品种,可作为优良建兰品种进行种植推广。

Seventeen varieties of Cymbidium ensifolium were used as experimental materials to analyze their photosynthetic characteristics.The chlorophyll was extracted by modified acetone method,and then the photosynthetic pigment content was calculated by the Arnon acetone method formula.The chlorophyll fluorescence parameters were measured by the Czech FluorCam open chlorophyll fluorometer.The results showed that the photosynthetic pigments and chlorophyll fluorescence parameters of 17 C.ensifolium varieties showed different degrees of difference.Among them,the content of photosynthetic pigment parameters of C.ensifolium‘Tiegusu’,C.ensifolium‘Yihongshuangjiao’and C.ensifolium‘Minnanhuangdie’were higher than other varieties,indicating that these three varieties had good photosynthetic efficiency and strong ability to absorb light energy.In addition,the maximum fluorescence yield(Fm),Kautsky effect maximum fluorescence(Fp),PSⅡoriginal light energy conversion efficiency(Fv/Fm)and non-photochemical quenching coefficient(NPQ)of‘Tiegusu’were higher than those of other varieties.In summary,‘Tiegusu’had the best photosynthetic and physiological characteristics and could be promoted as an excellent planting resource.