茉莉茎段遗传转化体系初步研究

Preliminary Study of Genetic Transformation System of Jasmine Stem Segment

以双瓣茉莉(Jasminum sambac)一年生茎段为外植体,探索茉莉花遗传转化体系。结果显示,预培养7 d后进行农杆菌侵染,然后置于含卡那霉素(Kan)80~100 mg·L^-1的筛选培养基上培养可获得成活率较高的抗性茎段。GFP转化预培养7 d的茉莉茎段再培养3 d及30 d后,以及转化预培养20 d茉莉茎段愈伤再培养14 d后愈伤组织中均能检测到GFP荧光。说明利用该转化系统进行茉莉遗传转化是可行的。

In this study,the genetic transformation system of jasmine was studied.The stem segment of the annual shoots of Jasminum sambac was used as the explants.The results showed that the resistant stem segments and callus with high survival rate could be obtained under the conditions that treating with agrobacterium infection after 7 d pre-culture,and then culturing on the screening medium with Kan concentration of 80 to 100 mg·L^-1.The GFP gene was transformed into jasmine stem segments and screened,and GFP fluorescence was detected in the stem segments that pre-cultured for 7 d and then screened for 3 d or 30 d after transformation,and also detected in the stem callus that pre-cultured for 20 d and screened for 14 d after transformation,indicating that GFP gene successfully transformed into jasmine stem segments.The results indicated that the transformation system was feasible,which laid a foundation for subsequent functional studies of jasmine genes.