福建省域内三叶青优良种源地选择研究

Excellent Provenance Selection of Tetrastigma hemsleyanum in Fujian Province

以福建省不同产地三叶青(Tetrastigma hemsleyanum)块根为材料,对比各地理种源三叶青块根总黄酮含量差异,并以总黄酮含量为指标进行系统聚类分析,探讨地理及气候因子与总黄酮含量的相关性。结果表明,不同种源的三叶青块根总黄酮含量有不同程度的差异,同一种源的三叶青块根总黄酮含量差异不显著。基于块根中的总黄酮含量可以将24个三叶青地理种源划分为三大类,第一类包括宁德周宁、宁德古田、三明大田、泉州永春、南平建瓯和三明永安等6个地理种源,第二类包含三明将乐、三明尤溪、龙岩连城和福州永泰等4个地理种源,第三类包含龙岩新罗、福州闽侯、南平延平、泉州德化、漳州南靖、龙岩永定、厦门同安、龙岩武平、莆田仙游、南平光泽、南平顺昌、三明沙县、南平浦城和漳州长泰等14个地理种源。其中第一类种源三叶青总黄酮含量平均为28.77 mg·g^-1,较全省平均水平高60.10%。三叶青总黄酮含量与种源地海拔呈极显著正相关,与种源地年平均气温呈极显著负相关,与年平均降水量呈显著正相关,与无霜期呈显著负相关,与经纬度之间的相关性则不显著。

In this study,the roots of Tetrastigma hemsleyanum from different geographical provenances in Fujian were used as materials,and the total flavonoid contents in the roots were compared.The total flavonoids content in the roots was used as an indicator to perform systematic clustering,and the correlation between geography,climate factors and total flavonoids content was explored.The results showed that the total flavonoid contents in the roots of T.hemsleyanum from different provenances were extremely different,and the total flavonoids content in the roots of T.hemsleyanum from the same source were not significantly different.The 24 geographical provenances of Tetrastigma hemsleyanum could be divided into 3 categories based on the total flavonoids content in the roots.The first category includes 6 geographical provenances:Ningde Zhouning,Ningde Gutian,Sanming Datian,Quanzhou Yongchun,Nanping Jian’ou,and Sanming Yong’an.The second category includes 4 geographical provenances:Sanming Jiangle,Sanming Youxi,Longyan Liancheng,Fuzhou Yongtai.The third category includes 14 geographical provenances:Longyan Xinluo,Fuzhou Minhou,Nanping Yanping,Quanzhou Dehua,Zhangzhou Nanjing,Longyan Yongding,Xiamen Tong’an,Longyan Wuping,Putian Xianyou,Nanping Guangze,Nanping Shunchang,Sanming Sha County,Nanping Pucheng,Zhangzhou Changtai.Among them,the average content of total flavonoids of Tetrastigma hemsleyanum from the first type of provenance was 28.77 mg·g-1,which was 60.10%higher than the provincial average.The total flavonoids content of Tetrastigma hemsleyanum had a very significant positive correlation with the provenance altitude,a very significant negative correlation with the provenance annual average temperature,a significant positive correlation with the annual average precipitation,and a significant negative correlation with the frost-free period,but no significant correlation with longitude and latitude.