摘要
目的:检测某院37株多重耐药鲍曼不动杆菌的同源性,发现医院感染防控缺陷,为制定医院感染管理方案提供依据。方法:收集2015-2016年在某院分离的非重复多重耐药鲍曼不动杆菌37株,采用下一代测序技术检测其基因序列,用构树软件MEGA构建进化树,分析其同源关系,并按同源关系分组:结果:37株多重耐药鲍曼不动杆菌测序完成并构建进化树,按同源性分成5组。组内同源性较高:结论:37株多重耐药鲍曼不动杆菌组内同源性较高,具有院内交叉感染。
Objective:This study was carried out to detect the homology of 37 multidrug-resistant acinetobacter baumannii in a hospital and find the defects in prevention and control of nosocomial infection,so as to provide a basis for formulating hospital infection management plan.Method:37 strains of non-repetitive multidrug-resistant acinetobacter baumannii were isolated and collected in a hospital from 2015 to 2016.Their gene sequences were detected by next-generation sequencing technology.Evolutionary trees were constructed by the tree construction software MEGA,and their homology were analyzed and grouped according to the homology relationship.Result:37 strains of multidrug-resistant acinetobacter baumannii were sequenced and an evolutionary tree was constructed.According to the homology,they were divided into 5 groups,with high homology within the group.Conclusion:37 strains of multidrug-resistant acinetobacter baumannii have high homology in the group,and there is a possibility of cross-infection in the hospital.
作者
程模
吴朝菊
张露
何霖
杨培洪
CHENG Mo;WU Chao-ju;ZHANG Lu;HE Lin;YANG Pei-hong(Department of Pharmacy,Dianjiang County People's Hospital,Dianjiang 408300,Chongqing;Deparment of Laboratory.Dianjiang County People's Hospital,Dianjiang 408300,Chongqing)
出处
《中药与临床》
2020年第5期47-50,共4页
Pharmacy and Clinics of Chinese Materia Medica
基金
重庆市卫计委医学科研计划项目(编号:2017MSXM193)。
关键词
鲍曼不动杆菌
下一代测序技术
同源性检测
医院感染
Acinetobacter baumannii
next-generation sequencing technology
homology detection
hospital infection