IL-18和ESM-1在急性呼吸窘迫综合征大鼠中的作用

Role of IL-18 and ESM-1 in acute respiratory distress syndrome rats

目的探讨白细胞介素18(IL-18)和内皮细胞特异分子1(ESM-1)在急性呼吸窘迫综合征(ARDS)大鼠的作用。方法健康清洁级雄性大鼠120只(5周),完全数字随机分为正常对照组、干预组(气管滴定生理盐水)和ARDS组(气管滴定脂多糖内毒素),每组各40只。实时RT-PCR检测各组大鼠左下肺6、12、24、48 h的IL-18 mRNA和ESM-1 mRNA表达。酶联免疫吸附试验双抗体夹心法测定各组大鼠不同时间点的血清IL-18和ESM-1含量。免疫组织化学检测各组大鼠左下肺不同时间点的IL-18 mRNA和核因子κB mRNA表达。同样取各组大鼠右肺下叶供HE染色病理观察。结果光镜下干预组大鼠未出现明显的病理变化;ARDS组大鼠肺泡间质增宽、肺泡壁增厚、肺泡结构破坏,肺泡腔消失。ARDS组各时间点的IL-18 mRNA和ESM-1 mRNA表达与干预组比较,差异均有统计学意义(P值均<0.01);ARDS组6 h IL-18 mRNA和ESM-1 mRNA与48 h比较,差异有统计学意义(P值均<0.05);ARDS组各时间点的血清IL-18和ESM-1含量均高于干预组(P值均<0.01),ARDS组6 h IL-18和ESM-1含量与48 h比较,差异均有统计学意义(P值均<0.05)。结论IL-18和ESM-1可以用于ARDS早期诊断和病情评估。

Objective To discuss the mechanism of IL-18 and ESM-1 in acute respiratory distress syndrome(ARDS)rats.Methods ARDS rat model was established by tracheal titration lipopolysaccharide endotoxin,physiological saline volume tracheal titration as normal control group.120 clean grade rats rats were completely randomly divided into normal control group,ARDS model group 6 h,12 h,24 h,48 h.12 groups of rats left lower lung interleukin-18(IL-18)mrna and endothelial cell-specific molecule 1(ESM-1)expression with real-time rt-pcr detection.Taking each group rats serum clamp by double antibody ELISA method determine each group rats serum IL-18 and ESM-1 content and by immunohistochemical determine five groups of rats with left lower lung IL-18 and nuclear factor kappa B expression.Also taking groups of rats right pulmonary lower lobe for pathological are observated by HE staining.Results The normal control group rats do not appear obvious pathological changes,then the ARDS model group rats are alveolar interstitial thickening,alveolar edema,alveolar structure and alveolar space disappeared,inflammatory cells exudated,pulmonary fibrosis formated by light microscopy.IL-18 mRNA and ESM-1 mRNA in ARDS model are different compared with the control group respectively(all P<0.01);IL-18 mRNA and ESM-1 mRNA in ARDS model 6 h and 48 h were significant(both P<0.05);the contents of serum IL-18 and ESM-1 in the 4 groups of ARDS models were higher than the control group(all P<0.01),and the contents of IL-18 and ESM-1 in 48 h ARDS model were different,respectively than ARDS model 6 h(both P<0.05).Conclusions IL-18 and ESM-1 can be used for ARDS in early diagnosis and condition evaluation.