老翘的化学成分研究

Chemical constituents of grown Fructus Forsythiae

对老翘进行化学成分和细胞毒活性的研究。利用硅胶柱色谱、SepHadex LH-20柱色谱、ODS柱色谱和半制备HPLC等多种方法进行分离纯化,通过1H NMR、13 C NMR、ESI-MS等方法解析化合物的结构。共分离鉴定得到了17个化合物,分别鉴定为五福花苷酸(1)、连翘苷(2)、芦丁(3)、(+)-松脂素-4-O-β-D-吡喃葡萄糖苷(4)、(+)-表松脂素-β-D-吡喃葡萄糖苷(5)、(+)-羟基表松脂素-4′′-β-D-葡萄糖(6)、连翘脂苷A(7)、连翘新苷(8)、木通苯乙醇苷B(9)、(7 R,8 S,7′R,8′S)-8-hydroxypinoresinol-4-O-β-D-glucopyranoside(10)、木通苯乙醇苷A(11)、车前草苷A(12)、连翘酯苷E(13)、落叶松脂醇-4′-O-β-D-葡萄糖苷(14)、连翘酯苷G(15)、连翘酯苷H(16)、(+)松脂素单甲基醚-β-D-葡萄糖甙(17)。其中,化合物4、6、9、10、14、17均是首次从该属植物中分离得到。通过MTT法对17个单体化合物进行体外抗肿瘤活性筛选,活性测定结果表明化合物1~17均没有抑制肿瘤细胞增殖作用。

The chemical constituents and cytotoxic activity of grown Fructus Forsythiae were studied.The compounds were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography,ODS column chromatography and semi preparative HPLC.The structures of the compounds were analyzed by 1H NMR,13 C NMR and ESI-MS.Seventeen compounds were identified as adoxosidic acid(1),phillyrin(2),rutin(3),(+)-pinoresinol-4-O-β-D-glucoside(4),(+)-epipinoresinol-β-D-glucoside(5),(+)-rpipinoresinol-4′′-β-D-glucoside(6),forsythoside A(7),lianqiaoxinside(8),calceolariolside B(9),(7 R,8 S,7′R,8′S)-8-hydroxypinoresinol-4-O-β-D-glucopyranoside(10),calceolarioside A(11),plantainoside A(12),forsythoside E(13),lariciresinol 4′-O-β-D-glucoside(14),forsythoside G(15),forsythoside H(16),(+)-pinoresinolmonomethyether-β-D-glucoside(17).Compounds 4,6,9,10,14,17 were isolated from this genus for the first time.The antitumor activities of 17 compounds were screened by MTT assay.The results showed that compounds 1-17 did not inhibit the proliferation of tumor cells.