粪肠球菌和变异链球菌共培养双菌生物膜性质的初步研究

Preliminary Study on Dual-species Biofilm of Enterococcus faecalis and Streptococcus mutans

目的:研究变异链球菌UA140-mrfp和粪肠球菌ATCC 29212的双菌生物膜形成量和在微流体系统(BioFlux System)中双菌生物膜的结构。方法:变异链球菌UA140-mrfp和粪肠球菌ATCC 29212混合培养后,结晶紫染色检测生物膜量;BioFlux200系统中接种变异链球菌UA140-mrfp和粪肠球菌ATCC 29212至观察区,静置30 min后,在0.8 dyne(达因)剪切力作用下连续培养12 h。在荧光显微镜下观察生物膜结构。结果:变异链球菌和粪肠球菌双菌生物膜量和单菌相比无统计学差异,但在微流体环境下能够形成具有网状分布的双菌生物膜。结论:使用BioFlux微流体系统,能够培养具有三维结构的变异链球菌和粪肠球菌双菌生物膜。

Objective:To measure the biomass and observe the structure of dual-species biofilm of Streptococcus mutans UA140-mrfp and Enterococcus faecalis ATCC 29212 using BioFlux System.Methods:Biofilm volume was measured by crystalline violet staining after mixed culture of Streptococcus mutans and Enterococcus faecalis.After 30 min standing,dual-species biofilm was cultured in BioFlux200 system under 0.8 dyne continuous shear force for 12 hours.The biofilm structure was observed under fluorescence microscope.Results:The volume of dual-species biofilm was not statistically different from that of mono-species biofilm.However,the dual-species biofilm could form reticulated distribution biofilms in microfluidic environment.Conclusion:Compared with static biofilm models,BioFlux system could form integrated dual-species biofilm.