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组蛋白甲基化转移酶2在糖尿病肾病小鼠肾组织中的表达变化及其意义 被引量:3

Expression and significance of histone methyltransferase SMYD2 in renal tissue of diabetic nephropathy mice and its expression in renal tissue of diabetic nephropathy mice
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摘要 目的检测组蛋白甲基化转移酶2(SET and MYND domain containing 2,SMYD2)在糖尿病小鼠肾组织中的表达变化,分析其与上皮细胞⁃间充质细胞转化(epithelial⁃mesenchymal transformation,EMT)的相关性,为临床上治疗糖尿病肾病(diabetic nephropathy,DN)提供新的方向。方法24只雄性C57BL/6小鼠随机分为正常对照(NC)组、DM12周组、DM24周组和DM28周组,每组6只。采用腹腔注射链脲佐菌素(streptozotocin,STZ)复制DM小鼠模型,处死小鼠后收集血清和肾组织,全自动生化分析仪测血糖(BG)、血肌酐(Scr)和尿素氮(BUN);利用HE和Masson染色进行肾脏病理分析;应用Western blot方法检测α⁃平滑肌肌动蛋白(α⁃SMA)、波形蛋白(Vimentin)、SMYD2、转化生长因子⁃β1(TGF⁃β1)和E⁃钙黏素(E⁃cadherin)的表达。结果与NC组相比,DM组小鼠BG、Scr和BUN水平均明显增加(P<0.05),24周组和28周组肾组织出现明显的病理改变和纤维增生,α⁃SMA、Vimentin、SMYD2和TGF⁃β1蛋白表达增加(P<0.05),E⁃cadherin蛋白表达减少(P<0.05),其中SMYD2与E⁃cadherin的表达呈负相关,与α⁃SMA,Vimentin和TGF⁃β1的表达呈正相关。结论SMYD2在DM小鼠肾组织中表达上调,推测其可能和TGF⁃β1表达上调共同参与EMT的发生,进而参与调控DN的发生发展。 Objective To detect the expression of SET and MYND domain containing 2(SMYD2)in renal tissue of diabetic mice and analyze its correlation with epithelial⁃mesenchymal transition(EMT),which provides a new direction for the clinical treatment of diabetic nephropathy.Methods Twenty⁃four male C57BL/6 mice were randomly assigned to normal control(NC)group,DM12 week group,DM24 week group and DM28 week group,6 mice in each group,were intraperitoneal injected with Streptozotocin⁃induced replication of DM mouse model.Mice were sacrificed at the corresponding time.Serum and renal tissue samples were collected.Blood glucose,serum creatinine and blood urea nitrogen were measured by biochemical methods.Pathological changes of renal tissue were observed after HE and Masson staining.The expression of⁃SMA,Vimentin,SMYD2,TGF⁃β1 and E⁃cadherin were detected by Western blot.Results Compared with NC group,blood glucose,serum creatinine and blood urea nitrogen of the diabetic group increased(P<0.05).Significant pathological changes and fibrous hyperplasia were observed in renal tissues at 24 weeks and 28 weeks.α⁃SMA,Vimentin,SMYD2 and TGF⁃β1 protein expression wassignificantly increased(P<0.05)at 24 weeks and 28 weeks,E⁃cadherin was decreased(P<0.05).The results of correlation analysis showed that SMYD2 was negatively correlated with E⁃cadherin expression and positively correlated withα⁃SMA,Vimentin and TGF⁃β1 expression.Conclusion SMYD2 expression is upregulated in renal tissue of DM mice,and it is speculated that SMYD2 may participate in the occurrence of EMT together with the up⁃regulation of TGF⁃β1 expression,and thus participate in the regulation of the occurrence and development of DN.
作者 简久莹 张妮 余婷 王笑笑 伍聪聪 徐卫卫 郭兵 刘丽荣 JIAN Jiuying;ZHANG Ni;YU Ting;WANG Xiaoxiao;WU Congcong;XU Weiwei;GUO Bing;LIU Lirong(Department of Clinical Hematology,Guizhou Medical University,Guiyang 550004,China;Clinical Laboratory Center,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;不详)
出处 《实用医学杂志》 CAS 北大核心 2020年第23期3194-3198,共5页 The Journal of Practical Medicine
基金 国家自然科学基金资助项目(编号:81860134) 贵州省高层次留学人才创新创业择优资助项目(编号:留学人才择优资助合同(2018)01号)。
关键词 组蛋白甲基化转移酶2 糖尿病肾病 上皮细胞⁃间充质细胞转化 转化生长因子⁃β1 SET and MYND domain containing 2 diabetic nephropathy epithelial⁃mesenchymal transformation transforming growth factor beta 1
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