脲酶--谷氨酸脱氢酶法快速测定黄酒及其发酵液中的尿素

Rapid determination of urea in chinese rice wine and its fermentation process by Urease-Glutamate Dehydrogenase method

建立了脲酶-谷氨酸脱氢酶测定黄酒及黄酒生产过程发酵液中尿素的分析方法。采用阳离子交换树脂除去黄酒中内源氨,消除了对尿素测定的干扰,采用脲酶-谷氨酸脱氢酶法对黄酒中尿素进行检测,尿素含量在2.0~50.0mg/L浓度范围内线性关系良好,加标回收率(n=3)在100.31%~109.14%之间,重复性(n=10)在2.76%~3.50%之间,本方法与液相色谱-荧光法测定结果对比,二者无显著性差异。该法具有样品前处理简单、特异性好、灵敏度高、测定速度快等优点,适用于黄酒企业对黄酒和黄酒酿造过程尿素含量的快速检测。适用于酿酒企业对葡萄酒产品和酿造过程的质量控制。

The endogenous ammonia in Chinese rice wine samples was removed by cation exchange resin,and the interference of endogenous ammonia on the determination of urea by Urease-Glutamate Dehydrogenase method was eliminated.The linear relationship was good when the urea content was 2.0-50.0 mg/L,the recoveries(n=3)were 100.31%-109.14%,and the repeatability(n=10)was 2.76%-3.50%.There was no significant difference between Urease-Glutamate Dehydrogenase method and High performance liquid chromatography with fluorescence method(HPLC-FLD).This method has the advantages of simple sample pretreatment,good specificity,high sensitivity and fast determination speed.The methad suitable for the rapid determination of urea content in rice wine and rice wine brewing process,and also suitable for the quality control of wine products and brewing process.