白念珠菌基因功能研究的策略

Strategies for gene function research of Candida albicans: review of literature

白念珠菌是临床最常见的致病真菌,共有约6100个基因,阐明其基因功能,尤其是致病性和耐药性相关基因的功能对发现抗真菌的新策略和新靶点有举足轻重的意义。白念珠菌基因功能研究的策略主要包括基因敲除和基因表达调控,近年来,白念珠菌基因功能研究的技术手段不断发展,本文就常用技术的发展进行综述,对相关技术存在的不足和发展前景也进行了分析。

Candida albicans is the most common fungal pathogen in clinic.The genome of C.albicans contains about 6100 genes.Revealing functions of these genes,especially those involved in virulence and drug resistance,are of great significance for the discovery of new antifungal strategies and targets.Strategies frequently used in gene function studies include gene knockout and gene expression regulation.In recent years,technical methods for gene function investigations of C.albicans continuously develop.In this article,we reviewed the development of common techniques and further analyzed the shortcomings and prospects of certain techniques.