山苍子油对斜纹夜蛾细胞增殖和凋亡的影响

Effect of Litsea Cubeba Essential Oil on Proliferation and Apoptosis of Spodoptera Litura Cell

研究山苍子油对离体培养的斜纹夜蛾(Spodoptera litura,SL)细胞的增殖和凋亡的影响,并初步确定山苍子油主要成分柠檬醛对细胞具有毒杀活性,诱导细胞凋亡.分别采用CCK、MTT法和流式细胞法研究不同浓度的山苍子油抑制SL细胞的活性,利用流式细胞法验证不同浓度的柠檬醛对SL细胞凋亡的影响.研究结果表明,山苍子油具有抑制SL细胞的增殖的作用,且随着山苍子油浓度的增大和处理时间的延长,SL细胞的存活率降低,当山苍子油浓度增大至0.015%时,SL细胞的存活率显著降低(P<0.01);当处理时间达到72 h后,SL细胞的存活率同样呈现显著降低.柠檬醛作为山苍子油的主要活性成分,对SL细胞亦具有明显的抑制增殖的作用,且成剂量正相关,当柠檬醛浓度大于0.015%时,SL细胞的存活率显著降低(P<0.01).山苍子油和柠檬醛均具有明显的促进SL细胞凋亡的作用,并呈剂量依赖关系,初步确定了柠檬醛是山苍子油促进SL细胞凋亡的主要活性成分,为山苍子油基新型植物源农药的开发与利用提供依据.

In order to investigate the effects of Litsea cubeba essential oil(LCEO)on the proliferation and apoptosis of Spodoptera litura(SL)cells cultured in vitro and preliminarily determine the main toxic activity component,the CCK,MTT assay and flow cytometry were used to study the inhibitory effects of different concentrations of LCEO on SL cells.The effect of different concentrations of citral on the apoptosis of SL cells was verified by flow cell method The results showed LCEO can inhibit the proliferation of SL cells,and with the increase of the concentration of LCEO and the prolongation of treatment time,the survival rate of SL cells decreased.When the concentration of LCEO was increased to 0.015%,the survival rate of SL cells was significantly lower(P<0.01).And the treatment time reached 72 h,the survival rate of SL cells decreased significantly.As the main active component of LCEO,citral has obvious inhibitory effect on SL cells,and the dose is positively correlated.In addition,the citral concentration was greater than 0.015%,the survival rate of SL cells was significantly decreased(P<0.01).It is indicated that LCEO and citral can effectively promote cell apoptosis and show dose dependence,and citral is the main active ingredient of LCEO to promote apoptosis of SL cells.So,it also provides a basis for the development and utilization of new plant-derived pesticides based on LCEO.