基于网络药理学探讨降压通脉方的作用机制

Study on the Action Mechanism of Jiangya Tongmai Formula Based on Network Pharmacology

目的:运用网络药理学理念和方法探讨名中医效方降压通脉方的可能靶点及作用机制,加深对名中医效方的理解。方法:通过检索ETCM及BATMAN-TCM数据库获取降压通脉方成分及预测靶基因;采用STRING、Cytoscape软件及插件构建网络并分析。利用DAVID工具对关键靶点进行基因本体论(GO)功能富集分析、KEGG通路富集分析以及疾病(DO)富集分析。结果:从降压通脉方中筛选出关键化合物112个,Hub靶标113个,模块29个。主要涉及在炎性反应、信号转导、免疫、糖脂代谢等生物学过程中的多条信号通路。结论:初步揭示了降压通脉方主要活性成分、靶点及作用机制,为进一步深入探讨其药理学作用提供了参考。

Objective:To investigate the possible action mechanism of Jiangya Tongmai Formula based on network pharmacology.Methods:By searching the ETCM and BATMAN-TCM databases,the components of Jiangya Tongmai Formula and the predicted target genes were obtained;the network was constructed and analyzed by STRING,Cytoscape software and plug-ins.DAVID tools was used to perform gene ontology(GO)function enrichment analysis,KEGG pathway enrichment analysis,and disease(DO)enrichment analysis on key targets.Results:From Jiangya Tongmai Formula,112 key compounds,113 Hub targets and 29 modules were screened.It mainly involved multiple signal pathways in the biological processes of inflammation,signal transduction,immunity,and glucose and lipid metabolism,and many other signal path.Conclusion:The main active ingredients,targets and mechanism of Jiangya Tongmai Formula are initially revealed,which provides a reference for further in-depth study of its pharmacological effects.